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Glucuronidation of 6 alpha-hydroxy bile acids by human liver microsomes
- Publication Year :
- 1987
-
Abstract
- The glucuronidation of 6-hydroxylated bile acids by human liver microsomes has been studied in vitro; for comparison, several major bile acids lacking a 6-hydroxyl group were also investigated. Glucuronidation rates for 6 alpha-hydroxylated bile acids were 10-20 times higher than those of substrates lacking a hydroxyl group in position 6. The highest rates measured were for hyodeoxy- and hyocholic acids, and kinetic analyses were carried out using these substrates. Rigorous product identification by high-field proton nuclear magnetic resonance and by electron impact mass spectrometry of methyl ester/peracetate derivatives revealed that 6-O-beta-D-glucuronides were the exclusive products formed in these enzymatic reactions. These results, together with literature data, indicate that 6 alpha-hydroxylation followed by 6-O-glucuronidation constitutes an alternative route of excretion of toxic hydrophobic bile acids.
- Subjects :
- Adult
Male
Glucuronosyltransferase
Magnetic Resonance Spectroscopy
medicine.drug_class
Glucuronidation
Glucuronates
Mass Spectrometry
Enzyme catalysis
Substrate Specificity
Bile Acids and Salts
chemistry.chemical_compound
medicine
Humans
Aged
Bile acid
biology
Chemistry
Deoxycholic acid
Cholic acid
Cholic Acids
General Medicine
Nuclear magnetic resonance spectroscopy
Kinetics
Biochemistry
Child, Preschool
biology.protein
Microsome
Microsomes, Liver
Female
Research Article
Deoxycholic Acid
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....57854fd0b1ce9599643d99205d25d59f