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Petit -High Pressure Carbon Dioxide stress increases synthesis of S -Adenosylmethionine and phosphatidylcholine in yeast Saccharomyces cerevisiae
- Source :
- Biophysical Chemistry. 231:79-86
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Petit-High Pressure Carbon Dioxide (p-HPCD) is a promising nonthermal technology for foods pasteurization. Cluster analysis of gene expression profiles of Saccharomyces cerevisiae exposed to various stresses exhibited that gene expression profile for p-HPCD stress (0.5 MPa, 25 °C) was grouped into a cluster including profiles for Sodium Dodecyl Sulfate and Roundup herbicide. Both are detergents that can disorder membrane structurally and functionally, which suggests that cell membrane may be a target of p-HPCD stress to cause cell growth inhibition. Through metabolomic analysis, amount of S-Adenosylmethionine (AdoMet) that is used as methyl donor to participate in phosphatidylcholine synthesis via phosphatidylethanolamine (PE) methylation pathway, was increased after p-HPCD treatment for 2 h. The key gene OPI3 encoding phospholipid methyltransferase that catalyzes the last two steps in PE methylation pathway was confirmed significantly induced by RT-PCR. Transcriptional expression of genes (MET13, MET16, MET10, MET17, MET6 and SAM2) related to AdoMet biosynthesis was also significantly induced. Choline as the PC precursor and ethanolamine as PE precursor in Kennedy pathway were also found increased under p-HPCD condition. We also found that amounts of most of amino acids involving protein synthesis were found decreased after p-HPCD treatment for 2 h. Moreover, morphological changes on cell surface were observed by scanning electron microscope. In conclusion, the effects of p-HPCD stress on cell membrane appear to be a very likely cause of yeast growth inhibition and the enhancement of PC synthesis could contribute to maintain optimum structure and functions of cell membrane and improve cell resistance to inactivation.
- Subjects :
- 0301 basic medicine
S-Adenosylmethionine
030103 biophysics
Saccharomyces cerevisiae Proteins
Saccharomyces cerevisiae
Biophysics
Biochemistry
Cell membrane
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Phosphatidylcholine
Gene expression
Pressure
medicine
Cluster Analysis
Metabolomics
Phosphatidylethanolamine
biology
Cell growth
Organic Chemistry
Carbon Dioxide
biology.organism_classification
Yeast
030104 developmental biology
medicine.anatomical_structure
chemistry
Biocatalysis
Microscopy, Electron, Scanning
Phosphatidylcholines
Phosphatidyl-N-Methylethanolamine N-Methyltransferase
Subjects
Details
- ISSN :
- 03014622
- Volume :
- 231
- Database :
- OpenAIRE
- Journal :
- Biophysical Chemistry
- Accession number :
- edsair.doi.dedup.....577ab7266a498c4fb1c01576a3fdc897