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Protective effects of adenoviral cardiotrophin-1 gene transfer on rubrospinal neurons after spinal cord injury in adult rats
Protective effects of adenoviral cardiotrophin-1 gene transfer on rubrospinal neurons after spinal cord injury in adult rats
- Source :
- Neurotoxicity research. 5(7)
- Publication Year :
- 2004
-
Abstract
- Cardiotrophin-1 (CT-1), a muscle-derived cytokine, supports the survival of motoneurons in vivo and in vitro. The present study investigated whether adenoviral huCT-1 gene transfer protected injured neurons from cell death or atrophy and promoted regeneration of rubrospinal tract (RST) after spinal cord injury in adult rats. Administration of the adenoviral CT-1 vector (Adv-CT1) to C3-4 lateral funiculus hemisection cavity, that completely interrupted RST, led to sustained CT-1 expression. Providing Adv-CT1, which rescued 20% of neurons, could prevent the loss of injured rubrospinal neurons 8 weeks post-injury. Retrograde tracing with FluoroGold showed that 1.2% of RST neurons regenerated at least two segments caudal to the injury site. Anterograde tracing with biotinylated dextran amine revealed that the RST axons terminated in white matter and gray matter. Behavioral testing revealed a significant functional recovery in limb usage. This observation indicated that adenoviral CT-1 gene transfer into the injured cord promoted survival and regeneration of rubrospinal neurons in adult rats.
- Subjects :
- Pathology
medicine.medical_specialty
Heterozygote
Stilbamidines
Red nucleus
Cell Survival
Biotin
Biology
Toxicology
Adenoviridae
Nerve Fibers
medicine
Animals
Transgenes
Rats, Wistar
Spinal cord injury
Spinal Cord Injuries
Fluorescent Dyes
Red Nucleus
Neurons
Biotinylated dextran amine
Behavior, Animal
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Gene Transfer Techniques
Dextrans
medicine.disease
Spinal cord
Retrograde tracing
Immunohistochemistry
Rats
Anterograde tracing
medicine.anatomical_structure
Spinal Cord
Cytokines
Female
Lateral funiculus
Neuroscience
Rubrospinal tract
Subjects
Details
- ISSN :
- 10298428
- Volume :
- 5
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Neurotoxicity research
- Accession number :
- edsair.doi.dedup.....574b386a5dbd1b970bdadc3679a6d22f