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Dorsal raphe serotonergic neurons promote arousal from isoflurane anesthesia

Dorsal raphe serotonergic neurons promote arousal from isoflurane anesthesia

Authors :
Qianzi Yang
Ao Li
Huihui Li
Xinxin Zhang
Rui Li
Peng-Rong Ouyang
Guangchao Zhao
Sa Wang
Hailong Dong
Zhenghua Zhu
Haopeng Zhang
Dan Wang
Mingzi Ran
Source :
CNS Neuroscience & Therapeutics
Publication Year :
2021
Publisher :
Wiley, 2021.

Abstract

Aims General anesthesia has been widely applied in surgical or nonsurgical medical procedures, but the mechanism behind remains elusive. Because of shared neural circuits of sleep and anesthesia, whether serotonergic system, which is highly implicated in modulation of sleep and wakefulness, regulates general anesthesia as well is worth investigating. Methods Immunostaining and fiber photometry were used to assess the neuronal activities. Electroencephalography spectra and burst‐suppression ratio (BSR) were used to measure anesthetic depth and loss or recovery of righting reflex to indicate the induction or emergence time of general anesthesia. Regulation of serotonergic system was achieved through optogenetic, chemogenetic, or pharmacological methods. Results We found that both Fos expression and calcium activity were significantly decreased during general anesthesia. Activation of 5‐HT neurons in the dorsal raphe nucleus (DRN) decreased the depth of anesthesia and facilitated the emergence from anesthesia, and inhibition deepened the anesthesia and prolonged the emergence time. Furthermore, agonism or antagonism of 5‐HT 1A or 2C receptors mimicked the effect of manipulating DRN serotonergic neurons. Conclusion Our results demonstrate that 5‐HT neurons in the DRN play a regulative role of general anesthesia, and activation of serotonergic neurons could facilitate emergence from general anesthesia partly through 5‐HT 1A and 2C receptors.<br />General anesthesia decreases the activities of serotonergic neurons in the dorsal raphe. Activation of DRN 5‐HT neurons reduced the depth of anesthesia and accelerated the emergence from general anesthesia, partially through the 5‐HT 1A and 2C receptors.

Details

ISSN :
17555949 and 17555930
Volume :
27
Database :
OpenAIRE
Journal :
CNS Neuroscience & Therapeutics
Accession number :
edsair.doi.dedup.....573787e6057f1949c6ffbd57e79dafc9
Full Text :
https://doi.org/10.1111/cns.13656