Additional file 1 of An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome

Additional file 1. Figure S1. Analysis of the ubiquitinated site-containing proteins in rice from published studies. Figure S2. Location of all E3 ligase encoding genes on the rice chromosome. Figure S3. Different types of ubiquitin E3 ligase-encoding genes in rice and the number of RT-PCR cloned and chemically synthesized E3 genes in this study. Figure S4. Confirmation of the interaction between OsUBC14 and its candidate E3s. Figure S5. E3 ubiquitin ligase activity of OsRING77, OsRING113, OsPUB28, OsPUB46, OsPUB49 and OsPUB69 in vitro. Figure S6. Ubiquitination assay of GST-OsSKIPa by MBP-OsPUB46. Figure S7. E3 ubiquitin ligase activity of OsRFPH2-10, P3IP1 and OsRING336 in vitro. Figure S8. The E3 ubiquitin ligase activity of OsRING116 in vitro and ubiquitination analysis of rTGA2.1. Figure S9. Transcript level of OPAL1 in individual overexpression lines and the editing types of OsFBK16. Figure S10. Degradation assay of OsPAL5 and OsPAL6 by OsFBK16 in vivo. Figure S11. Transcript level of OsPAL6 in individual overexpression lines.