Back to Search
Start Over
Monoclonal Antibodies against Nucleophosmin Mutants: Potentials for the Detection of Acute Myeloid Leukemia
- Source :
- International Journal of Medical Sciences
- Publication Year :
- 2011
- Publisher :
- Ivyspring International Publisher, 2011.
-
Abstract
- Nucleophosmin (NPM1) gene mutations resulting in cytoplasmic delocalization of Nucleophosmin (NPMc+) are the most common genetic alteration in acute myeloid leukemia (AML). Here, we attempted to prepare monoclonal antibodies (mAbs) against NPM1 mutation A (NPM-mA) and investigated the mAbs' clinical utility in immunohistochemical detection of NPMc+AML. The pET-32a-NPM-mA vector with the whole open reading frame of the NPM-mA gene was constructed. E.coli BL21 transformed with the vector were induced to express the NPM-mA recombinant protein. BALB/c mice were immunized with the recombinant NPM-mA. Positive clones were selected by indirect ELISA and the mAbs were obtained. Immunohistochemistry was performed to detect the NPMc+ in bone marrow smears from 10 AML patients with NPM-mA. The results showed that the pET-32a-NPM-mA vector was successfully constructed and the NPM-mA recombinant protein was used to immunize the mice. Two positive clones (2G3 and 3F9) were selected. The mAbs against NPM-mA were raised, but did cross-react with wild type NPM1. The mAbs can be used to detect the cytoplasmic dislocation of NPM1 in all AMLs carrying NPM-mA. Our results show that anti-NPM-mA mAbs were produced. Though they would cross-react with wild type NPM1, the mAbs may still have potential in the detection of NPMc+AMLs.
- Subjects :
- NPM1
nucleophosmin mutants
medicine.drug_class
Gene mutation
Biology
Monoclonal antibody
law.invention
Mice
law
hemic and lymphatic diseases
medicine
Animals
Humans
acute leukemia
Nucleophosmin
Acute leukemia
Mice, Inbred BALB C
integumentary system
Wild type
Myeloid leukemia
Antibodies, Monoclonal
Nuclear Proteins
General Medicine
Virology
Molecular biology
Immunohistochemistry
Recombinant Proteins
Leukemia, Myeloid, Acute
monoclonal antibody
Mutation
Recombinant DNA
Female
recombinant protein
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 14491907
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- International Journal of Medical Sciences
- Accession number :
- edsair.doi.dedup.....5727206340ccfc12e132dcb8155b7550