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Synchrotron radiation microtomography of brain hemisphere and spinal cord of a mouse model of multiple sclerosis revealed a correlation between capillary dilation and clinical score
- Source :
- Journal of Comparative Neurology. 527:2091-2100
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Multiple sclerosis is a neurological disorder in which the myelin sheaths of axons are damaged by the immune response. We report here a three-dimensional structural analysis of brain and spinal cord tissues of a mouse model of multiple sclerosis, known as experimental autoimmune encephalomyelitis (EAE). EAE-induced mice were raised with or without administration of fingolimod, which is used in the treatment of multiple sclerosis. Brains and spinal cords dissected from the EAE mice were lyophilized so as to reconstitute the intrinsic contrast of tissue elements, such as axons, in X-ray images. Three-dimensional structures of the brain hemispheres and spinal cords of the EAE mice were visualized with synchrotron radiation microtomography. Microtomographic cross sections reconstructed from the X-ray images revealed dilation of capillary vessels and vacuolation in the spinal cord of the EAE mice. Vacuolation was also observed in the cerebellum, suggesting that the neuroinflammatory response progressed in the brain. The vessel networks and vacuolation lesions in the spinal cords were modelled by automatically tracing the three-dimensional image in order to analyze the tissue structures quantitatively. The results of the analysis indicated that the distribution of vacuolations was not uniform but three-dimensionally localized. The mean vessel diameter showed a linear correlation with the clinical score, indicating that vasodilation is relevant to paralysis severity in the disease model. We suggest that vasodilation and vacuolation are related with neurological symptoms of multiple sclerosis.<br />22 pages, 5 figures, 1 table
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Cerebellum
Encephalomyelitis, Autoimmune, Experimental
Multiple Sclerosis
FOS: Physical sciences
Vasodilation
Neurological disorder
Mice
03 medical and health sciences
Imaging, Three-Dimensional
0302 clinical medicine
Paralysis
medicine
Animals
Physics - Biological Physics
business.industry
General Neuroscience
Multiple sclerosis
Experimental autoimmune encephalomyelitis
Brain
X-Ray Microtomography
Spinal cord
medicine.disease
Physics - Medical Physics
Fingolimod
Capillaries
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Spinal Cord
Biological Physics (physics.bio-ph)
Medical Physics (physics.med-ph)
medicine.symptom
business
Synchrotrons
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- ISSN :
- 10969861 and 00219967
- Volume :
- 527
- Database :
- OpenAIRE
- Journal :
- Journal of Comparative Neurology
- Accession number :
- edsair.doi.dedup.....571939dd6f58d32e437443638ec4136f