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Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-β-xylanase activity
- Source :
- Biotechnology for Biofuels, Vol 10, Iss 1, Pp 1-17 (2017), Biotechnology for Biofuels, Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2017
- Publisher :
- BMC, 2017.
-
Abstract
- Background Using globally abundant crop residues as a carbon source for energy generation and renewable chemicals production stand out as a promising solution to reduce current dependency on fossil fuels. In nature, such as in compost habitats, microbial communities efficiently degrade the available plant biomass using a diverse set of synergistic enzymes. However, deconstruction of lignocellulose remains a challenge for industry due to recalcitrant nature of the substrate and the inefficiency of the enzyme systems available, making the economic production of lignocellulosic biofuels difficult. Metatranscriptomic studies of microbial communities can unveil the metabolic functions employed by lignocellulolytic consortia and identify novel biocatalysts that could improve industrial lignocellulose conversion. Results In this study, a microbial community from compost was grown in minimal medium with sugarcane bagasse sugarcane bagasse as the sole carbon source. Solid-state nuclear magnetic resonance was used to monitor lignocellulose degradation; analysis of metatranscriptomic data led to the selection and functional characterization of several target genes, revealing the first glycoside hydrolase from Carbohydrate Active Enzyme family 11 with exo-1,4-β-xylanase activity. The xylanase crystal structure was resolved at 1.76 Å revealing the structural basis of exo-xylanase activity. Supplementation of a commercial cellulolytic enzyme cocktail with the xylanase showed improvement in Avicel hydrolysis in the presence of inhibitory xylooligomers. Conclusions This study demonstrated that composting microbiomes continue to be an excellent source of biotechnologically important enzymes by unveiling the diversity of enzymes involved in in situ lignocellulose degradation. Electronic supplementary material The online version of this article (10.1186/s13068-017-0944-4) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Crop residue
lcsh:Biotechnology
Biomass
Bioethanol
Management, Monitoring, Policy and Law
Biology
engineering.material
Applied Microbiology and Biotechnology
lcsh:Fuel
03 medical and health sciences
lcsh:TP315-360
lcsh:TP248.13-248.65
Hydrolase
Microbial community
Glycoside hydrolase
Food science
Metatranscriptomics
2. Zero hunger
Renewable Energy, Sustainability and the Environment
business.industry
Compost
Xylanase
Research
CELULOSE
fungi
Biotechnology
030104 developmental biology
General Energy
13. Climate action
Biofuel
engineering
business
Bagasse
Lignocellulose
Subjects
Details
- Language :
- English
- ISSN :
- 17546834
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biotechnology for Biofuels
- Accession number :
- edsair.doi.dedup.....570dcd287d71d89bceaf15f74335b74f