Back to Search
Start Over
Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Portfolio, 2020.
-
Abstract
- The global burden of bone-related diseases is increasing in the aging society; thus, improved bone targeted imaging for their early identification and treatment are needed. In this study, we screened novel peptide ligands for hydroxyapatite, a major inorganic component of teeth and bones, and identified a peptide enabling in vivo bone targeting and real-time fluorescence bone detection. To isolate peptides highly specific for hydroxyapatite, we used negative and positive selection from a randomized 8-mer peptide phage library and identified hydroxyapatite-specific peptides (HA-pep2, HA-pep3, and HA-pep7). Among these three peptides, HA-pep3 showed the highest binding capacity and superior dissociation constant towards hydroxyapatite surfaces over time (~ 88.3% retained on hydroxyapatite after two weeks). Furthermore, HA-pep3 was highly specific for hydroxyapatite compared to other calcium salt-based materials. Using this superior specificity, HA-pep3 showed higher accumulation in skull, spine, and joints in comparison with scrambled control peptide during real-time whole-body imaging. Ex vivo analysis of the major organs and bone from mice demonstrated that the fluorescence intensity in bone was about 3.32 folds higher in the case of HA-pep3 than the one exhibited by the scrambled control peptide. Our study identified a novel approach for targeting ligands for bone specific imaging and can be useful for drug delivery applications.
- Subjects :
- Male
0301 basic medicine
Science
chemistry.chemical_element
Peptide
02 engineering and technology
Calcium
Biochemistry
Article
Bone and Bones
Mice
03 medical and health sciences
Drug Delivery Systems
Peptide Library
In vivo
medicine
Animals
Amino Acid Sequence
SPINE (molecular biology)
chemistry.chemical_classification
Mice, Inbred BALB C
Multidisciplinary
Molecular medicine
Chemistry
Optical Imaging
021001 nanoscience & nanotechnology
Chemical biology
Dissociation constant
Skull
Durapatite
030104 developmental biology
medicine.anatomical_structure
Drug delivery
Medicine
Peptides
Tomography, X-Ray Computed
0210 nano-technology
Ex vivo
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....57029a342726c58f150a054f4a9ea977