FIGURE 2 from Uncovering the Protective Neurologic Mechanisms of Hypofractionated FLASH Radiotherapy

Mice exposed to FLASH-RT performed similar to controls in hippocampal-dependent learning and memory tests OUL while CONV-RT mice did not. A, Objects in updated locations testing experimental design. B, Update session behavior. At 4 months post-irradiation, FLASH and control mice showed preference for the novel toy and location in both males (left) and females (right) while CONV did not. C, Updated information test session. CONV irradiated female mice failed to learn the updated novel (A4) object over its predecessor (A3) when compared with FLASH and control mice. CONV irradiated male mice performed significantly worse than FLASH mice. D, Original information test session. CONV irradiated female mice were unable to differentiate between the updated novel location (A4) and the original location (A1) while FLASH and control performed similarly. No significant changes in male mice were observed. All data were analyzed using a one-way ANOVA followed by Bonferroni multiple comparison test (n = 11–16/sex/treatment). *, P ≤ 0.05; **, P ≤ 0.01; ns, no significance.