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Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species
- Source :
- The Plant Journal. 96:1309-1316
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Chromosomal inversions occur in natural populations of many species, and may underlie reproductive isolation and local adaptation. Traditional methods of inversion discovery are labor-intensive and lack sensitivity. Here, we report the use of three-dimensional contact probabilities between genomic loci as assayed by chromosome-conformation capture sequencing (Hi-C) to detect multi-megabase polymorphic inversions in four barley genotypes. Inversions are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi-C as a generally applicable method for inversion discovery in natural populations.
- Subjects :
- 0301 basic medicine
Genotype
medicine.diagnostic_test
Chromosome Mapping
Hordeum
Genomics
Cell Biology
Plant Science
Reproductive isolation
Biology
Genome
Chromosomes, Plant
Chromosome conformation capture
03 medical and health sciences
030104 developmental biology
Evolutionary biology
Chromosome Inversion
Genetics
medicine
Hordeum vulgare
Genome, Plant
In Situ Hybridization, Fluorescence
Local adaptation
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 09607412
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- The Plant Journal
- Accession number :
- edsair.doi.dedup.....56d31eae7fc2a5a283f3bafff5d917b1