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Replicative phenotyping adds value to genotypic resistance testing in heavily pre-treated HIV-infected individuals--the Swiss HIV Cohort Study
- Source :
- Journal of Translational Medicine, Journal of Translational Medicine, Vol 9, Iss 1, p 14 (2011), Journal of Translational Medicine, vol. 9, pp. 14, Journal of Translational Medicine, Vol. 9 (2011) P. 14, Fehr, Jan; Glass, Tracy R; Louvel, Séverine; Hamy, François; Hirsch, Hans H; von Wyl, Viktor; Böni, Jürg; Yerly, Sabine; Bürgisser, Philippe; Cavassini, Matthias; Fux, Christoph A; Hirschel, Bernard; Vernazza, Pietro; Martinetti, Gladys; Bernasconi, Enos; Günthard, Huldrych F; Battegay, Manuel; Bucher, Heiner C; Klimkait, Thomas and Swiss HIV Cohort Study, (2011). Replicative phenotyping adds value to genotypic resistance testing in heavily pre-treated HIV-infected individuals--the Swiss HIV Cohort Study. Journal of translational medicine, 9, p. 14. London: BioMed Central 10.1186/1479-5876-9-14
- Publication Year :
- 2010
-
Abstract
- Background Replicative phenotypic HIV resistance testing (rPRT) uses recombinant infectious virus to measure viral replication in the presence of antiretroviral drugs. Due to its high sensitivity of detection of viral minorities and its dissecting power for complex viral resistance patterns and mixed virus populations rPRT might help to improve HIV resistance diagnostics, particularly for patients with multiple drug failures. The aim was to investigate whether the addition of rPRT to genotypic resistance testing (GRT) compared to GRT alone is beneficial for obtaining a virological response in heavily pre-treated HIV-infected patients. Methods Patients with resistance tests between 2002 and 2006 were followed within the Swiss HIV Cohort Study (SHCS). We assessed patients' virological success after their antiretroviral therapy was switched following resistance testing. Multilevel logistic regression models with SHCS centre as a random effect were used to investigate the association between the type of resistance test and virological response (HIV-1 RNA Results Of 1158 individuals with resistance tests 221 with GRT+rPRT and 937 with GRT were eligible for analysis. Overall virological response rates were 85.1% for GRT+rPRT and 81.4% for GRT. In the subgroup of patients with >2 previous failures, the odds ratio (OR) for virological response of GRT+rPRT compared to GRT was 1.45 (95% CI 1.00-2.09). Multivariate analyses indicate a significant improvement with GRT+rPRT compared to GRT alone (OR 1.68, 95% CI 1.31-2.15). Conclusions In heavily pre-treated patients rPRT-based resistance information adds benefit, contributing to a higher rate of treatment success.
- Subjects :
- Oncology
10028 Institute of Medical Virology
Male
Multivariate analysis
viruses
lcsh:Medicine
HIV Infections
Virus Replication
10234 Clinic for Infectious Diseases
Cohort Studies
0302 clinical medicine
Genotype
030212 general & internal medicine
Virus Replication/drug effects/physiology
Medicine(all)
ddc:616
0303 health sciences
Adult
Anti-HIV Agents/pharmacology
Anti-HIV Agents/therapeutic use
Dose-Response Relationship, Drug
Drug Resistance, Viral/physiology
Female
HIV Infections/diagnosis
HIV Infections/drug therapy
HIV-1/physiology
Humans
Microbial Sensitivity Tests/methods
Middle Aged
Phenotype
Predictive Value of Tests
Prognosis
Switzerland
Virus Replication/drug effects
Virus Replication/physiology
General Medicine
HIV Infections/diagnosis/drug therapy/virology
3. Good health
Predictive value of tests
Cohort study
medicine.medical_specialty
Anti-HIV Agents
610 Medicine & health
Microbial Sensitivity Tests
General Biochemistry, Genetics and Molecular Biology
Virus
03 medical and health sciences
1300 General Biochemistry, Genetics and Molecular Biology
Internal medicine
Drug Resistance, Viral
medicine
Anti-HIV Agents/pharmacology/therapeutic use
030304 developmental biology
Biochemistry, Genetics and Molecular Biology(all)
business.industry
Research
lcsh:R
Odds ratio
Institutional repository
Viral replication
Immunology
HIV-1
570 Life sciences
biology
business
Subjects
Details
- ISSN :
- 14795876
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of translational medicine
- Accession number :
- edsair.doi.dedup.....56c853a40301e43557c0adae370c3360