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Identification of transcriptional regulators in the mouse immune system
- Source :
- Nature immunology
- Publication Year :
- 2013
-
Abstract
- The differentiation of hematopoietic stem cells into cells of the immune system has been studied extensively in mammals, but the transcriptional circuitry that controls it is still only partially understood. Here, the Immunological Genome Project gene-expression profiles across mouse immune lineages allowed us to systematically analyze these circuits. To analyze this data set we developed Ontogenet, an algorithm for reconstructing lineage-specific regulation from gene-expression profiles across lineages. Using Ontogenet, we found differentiation stage-specific regulators of mouse hematopoiesis and identified many known hematopoietic regulators and 175 previously unknown candidate regulators, as well as their target genes and the cell types in which they act. Among the previously unknown regulators, we emphasize the role of ETV5 in the differentiation of γδ T cells. As the transcriptional programs of human and mouse cells are highly conserved, it is likely that many lessons learned from the mouse model apply to humans.
- Subjects :
- Cell type
Transcription, Genetic
T-Lymphocytes
Immunology
Computational biology
Biology
Article
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Gene expression
Immunology and Allergy
Animals
Humans
Cell Lineage
Gene Regulatory Networks
Gene
030304 developmental biology
Oligonucleotide Array Sequence Analysis
Genetics
0303 health sciences
Gene Expression Profiling
Cell Differentiation
Receptors, Antigen, T-Cell, gamma-delta
Genome project
DNA-Binding Proteins
Repressor Proteins
Haematopoiesis
Gene Expression Regulation
030220 oncology & carcinogenesis
Immune System
Trans-Activators
Identification (biology)
Stem cell
Transcriptome
Algorithms
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 15292916 and 15292908
- Volume :
- 14
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Nature immunology
- Accession number :
- edsair.doi.dedup.....56c3e19a0805c5f16906f4661b40e4f0