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Effects of yogurt and bifidobacteria supplementation on the colonic microbiota in lactose-intolerant subjects
- Source :
- Journal of Applied Microbiology, 104(2), 595-604. Wiley
- Publication Year :
- 2008
- Publisher :
- HAL CCSD, 2008.
-
Abstract
- Aims: Colonic metabolism of lactose may play a role in lactose intolerance. We investigated whether a 2-week supplementation of Bifidobacterium longum (in capsules) and a yogurt enriched with Bifidobacterium animalis could modify the composition and metabolic activities of the colonic microbiota in 11 Chinese lactose-intolerant subjects.Methods and Results: The numbers of total cells, total bacteria and the Eubacterium rectale/Clostridium coccoides group in faeces as measured with fluorescent in situ hybridization and the faecal beta-galactosidase activity increased significantly during supplementation. The number of Bifidobacterium showed a tendency to increase during and after supplementation. With PCR-denaturing gradient gel electrophoresis, in subjects in which B. animalis and B. longum were not detected before supplementation, both strains were present in faeces during supplementation, but disappeared after supplementation. The degree of lactose digestion in the small intestine and the oro-caecal transit time were not different before and after supplementation, whereas symptom scores after lactose challenge decreased after supplementation.Conclusions: The results suggest that supplementation modifies the amount and metabolic activities of the colonic microbiota and alleviates symptoms in lactose-intolerant subjects. The changes in the colonic microbiota might be among the factors modified by the supplementation which lead to the alleviation of lactose intolerance.Significance and Impact of the Study: This study provides evidence for the possibility of managing lactose intolerance with dietary lactose ( yogurt) and probiotics via modulating the colonic microbiota.
- Subjects :
- Male
Bifidobacterium longum
medicine.medical_treatment
polymerase chain reaction
Colony Count, Microbial
PROBIOTIC STRAIN
Lactose
Applied Microbiology and Biotechnology
[SDV.MP.PRB.BIF] Life Sciences [q-bio]/Microbiology and Parasitology/domain_sdv.mp.prb/domain_sdv.mp.prb.bif
chemistry.chemical_compound
Feces
Lactose Intolerance
fluids and secretions
Lactobacillus
Streptococcus thermophilus
Food science
LACTASE ACTIVITY
In Situ Hybridization, Fluorescence
Bifidobacterium
biology
TARGETED PROBES
16S RIBOSOMAL-RNA
food and beverages
General Medicine
Middle Aged
Yogurt
Bifidobacterium animalis
prebiotic
BACTERIA
SHORT-TERM
Electrophoresis, Polyacrylamide Gel
Female
ecology
HUMAN FECES
Biotechnology
Adult
China
Colon
digestive system
Statistics, Nonparametric
Lactase activity
GRADIENT GEL-ELECTROPHORESIS
Lactose Tolerance Test
medicine
Humans
FECAL MICROFLORA
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Clostridium
Lactose intolerance
Eubacterium
Prebiotic
Probiotics
[SDV.MP.PRB] Life Sciences [q-bio]/Microbiology and Parasitology/domain_sdv.mp.prb
IN-VITRO
biology.organism_classification
medicine.disease
beta-Galactosidase
chemistry
Dietary Supplements
dairy
metabolism
Biomarkers
Follow-Up Studies
Subjects
Details
- Language :
- English
- ISSN :
- 13645072
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Microbiology, 104(2), 595-604. Wiley
- Accession number :
- edsair.doi.dedup.....56c3a46fd4aea6ae4da9d6339f6c6006