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Determination of plasma amitriptyline by electron-capture gas chromatography after oxidation to anthraquinone
- Source :
- Journal of Chromatography A. 118:65-74
- Publication Year :
- 1976
- Publisher :
- Elsevier BV, 1976.
-
Abstract
- A selective procedure is described for the determination of amitriptyline in plasma. The method involves extraction, separation of amitriptyline from its metabolites and subsequent oxidation by ceric sulphate in 5.4 M sulphuric acid. The oxidation product, anthraquinone, is determined by means of electron-capture gas chromatography. The metabolites were separated by a column chromatographic extraction technique. The choice of oxidation reagent, optimum conditions for the oxidation, and the electron-capture properties of anthraquinone are discussed. The method can be used to determine down to 2 ng of amitriptyline in a plasma sample; the relative standard deviation at the 50-ng level was 4.0% (n = 8). The levels of amitriptyline found in a series of plasma samples are compared with those obtained by gas chromatography with use of nitrogen-specific detection; the two techniques gave coincident results.
- Subjects :
- Chromatography, Gas
Chromatography
Chemistry
Electron capture
Amitriptyline
Organic Chemistry
Extraction (chemistry)
Relative standard deviation
Anthraquinones
General Medicine
Plasma
Biochemistry
Anthraquinone
Analytical Chemistry
chemistry.chemical_compound
Reagent
Methods
medicine
Humans
Gas chromatography
Oxidation-Reduction
medicine.drug
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 118
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....56bb9b9e59452f88ac7a2cde55b85559