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Quantification of hair cortisol concentration in common marmosets (Callithrix jacchus) and tufted capuchins (Cebus apella)
- Source :
- American Journal of Primatology. 80:e22879
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Quantifying cortisol concentration in hair is a non-invasive biomarker of long-term hypothalamic-pituitary-adrenal (HPA) activation, and thus can provide important information on laboratory animal health. Marmosets (Callithrix jacchus) and capuchins (Cebus apella) are New World primates increasingly used in biomedical and neuroscience research, yet published hair cortisol concentrations for these species are limited. Review of the existing published hair cortisol values from marmosets reveals highly discrepant values and the use of variable techniques for hair collection, processing, and cortisol extraction. In this investigation we utilized a well-established, standardized protocol to extract and quantify cortisol from marmoset (n = 12) and capuchin (n = 4) hair. Shaved hair samples were collected from the upper thigh during scheduled exams and analyzed via methanol extraction and enzyme immunoassay. In marmosets, hair cortisol concentration ranged from 2,710 to 6,267 pg/mg and averaged 4,070 ± 304 pg/mg. In capuchins, hair cortisol concentration ranged from 621 to 2,089 pg/mg and averaged 1,092 ± 338 pg/mg. Hair cortisol concentration was significantly different between marmosets and capuchins, with marmosets having higher concentrations than capuchins. The incorporation of hair cortisol analysis into research protocols provides a non-invasive measure of HPA axis activity over time, which offers insight into animal health. Utilization of standard protocols across laboratories is essential to obtaining valid measurements and allowing for valuable future cross-species comparisons.
- Subjects :
- Male
0301 basic medicine
endocrine system
Hydrocortisone
Physiology
Article
03 medical and health sciences
0302 clinical medicine
biology.animal
medicine
Animals
Cebus
Ecology, Evolution, Behavior and Systematics
integumentary system
biology
Animal health
Marmoset
Callithrix
Upper thigh
biology.organism_classification
030104 developmental biology
medicine.anatomical_structure
Female
Animal Science and Zoology
Neuroscience research
hormones, hormone substitutes, and hormone antagonists
030217 neurology & neurosurgery
Hypothalamic–pituitary–adrenal axis
Hair
Subjects
Details
- ISSN :
- 02752565
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- American Journal of Primatology
- Accession number :
- edsair.doi.dedup.....56b5dbd909518d0643f35c59bf846334