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Wnt6 Plays a Complex Role in Maintaining Human Limbal Stem/Progenitor Cells
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-00273-y⟩, Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-00273-y⟩, Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021), Scientific reports, vol 11, iss 1
- Publication Year :
- 2021
- Publisher :
- Research Square Platform LLC, 2021.
-
Abstract
- The corneal epithelium is consistently regenerated by limbal stem/progenitor cells (LSCs), a very small population of adult stem cells residing in the limbus. Several Wnt ligands, including Wnt6, are preferentially expressed in the limbus. To investigate the role of Wnt6 in regulating proliferation and maintenance of human LSCs in an in vitro LSC expansion setting, we generated NIH-3T3 feeder cells to overexpress different levels of Wnt6. Characterization of LSCs cultured on Wnt6 expressing 3T3 cells showed that high level of Wnt6 increased proliferation of LSCs. Medium and high levels of Wnt6 also increased the percentage of small cells (diameter ≤ 12 µm), a feature of the stem cell population. Additionally, the percentage of cells expressing the differentiation marker K12 was significantly reduced in the presence of medium and high Wnt6 levels. Although Wnt6 is mostly known as a canonical Wnt ligand, our data showed that canonical and non-canonical Wnt signaling pathways were activated in the Wnt6-supplemented LSC cultures, a finding suggesting that interrelationships between both pathways are required for LSC regulation.
- Subjects :
- Cell Culture Techniques
Epithelium
WNT6
Mice
Cell growth
0302 clinical medicine
Stem Cell Research - Nonembryonic - Human
2.1 Biological and endogenous factors
Aetiology
Corneal epithelium
Adult stem cells
0303 health sciences
education.field_of_study
Multidisciplinary
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
Stem Cells
Wnt signaling pathway
Epithelium, Corneal
Cell Differentiation
Middle Aged
Cell biology
medicine.anatomical_structure
Differentiation
Medicine
Stem Cell Research - Nonembryonic - Non-Human
Adult stem cell
Cell signalling
Adult
animal structures
1.1 Normal biological development and functioning
Science
Population
Biology
Limbus Corneae
3T3 cells
Article
Cell Line
03 medical and health sciences
Underpinning research
Proto-Oncogene Proteins
medicine
Animals
Humans
Regeneration
Antigens
Progenitor cell
education
030304 developmental biology
Aged
Cell Proliferation
Corneal
Epithelial Cells
Stem Cell Research
Antigens, Differentiation
In vitro
Wnt Proteins
030221 ophthalmology & optometry
NIH 3T3 Cells
sense organs
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-021-00273-y⟩, Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-00273-y⟩, Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021), Scientific reports, vol 11, iss 1
- Accession number :
- edsair.doi.dedup.....56ab3b53038e5eb88c08069a59d9c279