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Glycosylation in Indolent, Significant and Aggressive Prostate Cancer by Automated High-Throughput N-Glycan Profiling
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 9233, p 9233 (2020), International Journal of Molecular Sciences, Volume 21, Issue 23
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The diagnosis and treatment of prostate cancer (PCa) is a major health-care concern worldwide. This cancer can manifest itself in many distinct forms and the transition from clinically indolent PCa to the more invasive aggressive form remains poorly understood. It is now universally accepted that glycan expression patterns change with the cellular modifications that accompany the onset of tumorigenesis. The aim of this study was to investigate if differential glycosylation patterns could distinguish between indolent, significant, and aggressive PCa. Whole serum N-glycan profiling was carried out on 117 prostate cancer patients&rsquo<br />serum using our automated, high-throughput analysis platform for glycan-profiling which utilizes ultra-performance liquid chromatography (UPLC) to obtain high resolution separation of N-linked glycans released from the serum glycoproteins. We observed increases in hybrid, oligomannose, and biantennary digalactosylated monosialylated glycans (M5A1G1S1, M8, and A2G2S1), bisecting glycans (A2B, A2(6)BG1) and monoantennary glycans (A1), and decreases in triantennary trigalactosylated trisialylated glycans with and without core fucose (A3G3S3 and FA3G3S3) with PCa progression from indolent through significant and aggressive disease. These changes give us an insight into the disease pathogenesis and identify potential biomarkers for monitoring the PCa progression, however these need further confirmation studies.
- Subjects :
- 0301 basic medicine
Glycan
Glycosylation
glycosylation
significant
Disease pathogenesis
medicine.disease_cause
Catalysis
Fucose
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
chemistry.chemical_compound
Prostate cancer
0302 clinical medicine
indolent
aggressive
medicine
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
chemistry.chemical_classification
biology
business.industry
Organic Chemistry
biomarkers
General Medicine
prostate cancer
medicine.disease
Computer Science Applications
carbohydrates (lipids)
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Glycan profiling
030220 oncology & carcinogenesis
Cancer research
biology.protein
Glycoprotein
Carcinogenesis
business
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....56911f66de3580d3946aa65766246cee