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PLGA based particles as 'drug reservoir' for antitumor drug delivery: characterization and cytotoxicity studies
- Source :
- Colloids and Surfaces B: Biointerfaces. 180:495-502
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Doxorubicin (DOX) is commonly used to treat several tumor types, but its severe side effects, primarily cardiotoxicity, represent a major limitation for its use in clinical settings. In this study we developed and characterized biodegradable and stable poly(D,L-lactic-co-glycolic) acid (PLGA) submicrocarriers employing an osmosis-based patented methodology, which allowed to optimize the drug loading efficiency up to 99%. Proceeding from this, we evaluated on MCF-7, a human breast cancer cell line, the ability of PLGA to promote the internalization of DOX and to improve its cytotoxicity in vitro. We found that the in vitro uptake efficiency is dramatically increased when DOX is loaded within PLGA colloidal carriers, which adhere to the cell membrane behaving as an efficient drug reservoir. In fact, the particles provide a diffusion-driven, sustained release of DOX across the cell membrane, resulting in high drug concentration. Accordingly, the cytotoxic analysis clearly showed that DOX-loaded PLGA exhibit a lower 50% inhibitory concentration than free DOX. The decay time of cell viability was successfully compared with DOX diffusion time constant from PLGA. The overall in vitro results highlight the potential of DOX-loaded PLGA particles to be employed as vectors with improved antitumor efficacy.
- Subjects :
- burst release
02 engineering and technology
01 natural sciences
Cell membrane
chemistry.chemical_compound
Drug Delivery Systems
Colloid and Surface Chemistry
Polylactic Acid-Polyglycolic Acid Copolymer
Anticancer
Burst release
Doxorubicin
Drug delivery
Internalization
PLGA carriers
polycyclic compounds
Cytotoxicity
Settore CHIM/02 - Chimica Fisica
media_common
Drug Carriers
Cell Death
010304 chemical physics
Chemistry
Surfaces and Interfaces
General Medicine
021001 nanoscience & nanotechnology
PLGA
medicine.anatomical_structure
MCF-7 Cells
0210 nano-technology
Biotechnology
medicine.drug
Drug
Cell Survival
media_common.quotation_subject
Antineoplastic Agents
macromolecular substances
drug delivery
anticancer
internalization
Hemolysis
Fluorescence
0103 physical sciences
medicine
Humans
Viability assay
Physical and Theoretical Chemistry
technology, industry, and agriculture
In vitro
carbohydrates (lipids)
Drug Liberation
Kinetics
Biophysics
Subjects
Details
- ISSN :
- 09277765
- Volume :
- 180
- Database :
- OpenAIRE
- Journal :
- Colloids and Surfaces B: Biointerfaces
- Accession number :
- edsair.doi.dedup.....5690a5b4eb66c3a6b462fc664cfea943