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Data on in vivo PGC-1alpha overexpression model via local transfection in aged mouse muscle
- Source :
- Data in Brief, Data in Brief, Vol 22, Iss, Pp 199-203 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- The data presented in this article are related to the research paper entitled “Intensified mitophagy in skeletal muscle with aging is downregulated by PGC-1alpha overexpression in vivo” (Yeo et al., 2019). The data explained the surgical procedure of in vivo local transfection by electroporation method in aged mouse tibialis anterior muscle, and plasmid DNA preparation and verification protocol. The data also showed the transfection efficiency levels of GFP or GFP-tagged PGC-1alpha through immunohistochemistry method for frozen muscle cross-sections.
- Subjects :
- 0303 health sciences
Multidisciplinary
Chemistry
Electroporation
fungi
Skeletal muscle
Transfection
lcsh:Computer applications to medicine. Medical informatics
Green fluorescent protein
Cell biology
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
Tibialis anterior muscle
In vivo
Biochemistry, Genetics and Molecular Biology
Mitophagy
medicine
lcsh:R858-859.7
Immunohistochemistry
lcsh:Science (General)
030217 neurology & neurosurgery
lcsh:Q1-390
030304 developmental biology
Subjects
Details
- ISSN :
- 23523409
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Data in Brief
- Accession number :
- edsair.doi.dedup.....5658022d4b6064a5d3936e8ea802bb14