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Coffee Improves Insulin-Stimulated Akt Phosphorylation in Liver and Skeletal Muscle in Diabetic KK-Ay Mice
- Source :
- Journal of Nutritional Science and Vitaminology. 58:408-414
- Publication Year :
- 2012
- Publisher :
- Center for Academic Publications Japan, 2012.
-
Abstract
- Coffee has an anti-diabetic effect, specifically the amelioration of both hyperglycemia and insulin resistance, in KK-A(y) mice, a type 2 diabetes animal model. To investigate coffee's effect on insulin signaling in liver, skeletal muscle, and adipose tissue (epididymal fat), we assayed the tyrosine phosphorylation of insulin receptor (IR) and serine phosphorylation of Akt. In Expt. 1, we assayed insulin signaling under nonfasting conditions in KK-A(y) mice that ingested water or coffee for 4 wk. Coffee ingestion ameliorated the development of hyperglycemia but did not affect insulin signaling in liver or skeletal muscle under such conditions. In Expt. 2, we assayed insulin signaling under basal and insulin-stimulated conditions in KK-A(y) mice that ingested water or coffee for 3 wk. The levels of tyrosine phosphorylation of insulin receptor in response to insulin injection in insulin-sensitive tissues were not different between mice that drank water and those that drank coffee. Coffee ingestion significantly increased the insulin-induced serine phosphorylation of Akt in liver and skeletal muscle, but not in epididymal fat, of KK-A(y) mice. Our results also indicated that coffee ingestion may contribute to the improvement of insulin resistance and hyperglycemia in KK-A(y) mice via the activation of Akt in insulin signaling in liver and skeletal muscle.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
medicine.medical_treatment
Medicine (miscellaneous)
Adipose tissue
Biology
Coffee
Diabetes Mellitus, Experimental
Mice
chemistry.chemical_compound
Insulin resistance
Internal medicine
Serine
medicine
Animals
Hypoglycemic Agents
Insulin
Phosphorylation
Muscle, Skeletal
Protein kinase B
Nutrition and Dietetics
Body Weight
Skeletal muscle
Tyrosine phosphorylation
medicine.disease
Receptor, Insulin
Insulin receptor
Endocrinology
medicine.anatomical_structure
Adipose Tissue
Liver
chemistry
Hyperglycemia
biology.protein
Tyrosine
Insulin Resistance
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 18817742 and 03014800
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Journal of Nutritional Science and Vitaminology
- Accession number :
- edsair.doi.dedup.....56572dcb3715b2a2f80b9e828df00603