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The polycomb group protein SUZ12 regulates histone H3 lysine 9 methylation and HP1 alpha distribution
- Source :
- Chromosome Research, Chromosome Res.
- Publication Year :
- 2006
-
Abstract
- Regulation of histone methylation is critical for proper gene expression and chromosome function. Suppressor of Zeste 12 (SUZ12) is a requisite member of the EED/EZH2 histone methyltransferase complexes, and is required for full activity of these complexes in vitro. In mammals and flies, SUZ12/Su(z)12 is necessary for trimethylation of histone H3 on lysine 27 (H3K27me3) on facultative heterochromatin. However, Su(z)12 is unique among Polycomb Group Proteins in that Su(z)12 mutant flies exhibit gross defects in position effect variegation, suggesting a role for Su(z)12 in constitutive heterochromatin formation. We investigated the role of Suz12 in constitutive heterochromatin and discovered that Suz12 is required for histone H3 lysine 9 tri-methylation (H3K9me3) in differentiated but not undifferentiated mouse embryonic stem cells. Knockdown of SUZ12 in human cells caused a reduction in H3K27me3 and H3K9me3, and altered the distribution of HP1α. In contrast, EZH2 knockdown caused loss of H3K27me3 but not H3K9me3, indicating that SUZ12 regulates H3-K9 methylation in an EZH2-independent fashion. This work uncovers a role for SUZ12 in H3-K9 methylation. © 2007 Springer. 15 299 314 Cited By :26
- Subjects :
- Chromosomal Proteins, Non-Histone
animal cell
Histone methylation
Histones
Mice
histone H3
Heterochromatin
Histone code
Tissue Distribution
EZH2
article
Polycomb Repressive Complex 2
Nuclear Proteins
protein function
unclassified drug
Neoplasm Proteins
DNA-Binding Proteins
female
priority journal
Histone methyltransferase
Mammalia
Polycomb protein
protein localization
macromolecular substances
Biology
Histone H3
heterochromatin protein 1
male
Histone H1
Histone H2A
Genetics
Animals
Humans
controlled study
Enhancer of Zeste Homolog 2 Protein
human
mouse
nonhuman
lysine
human cell
Lysine
fungi
Proteins
Histone-Lysine N-Methyltransferase
DNA Methylation
embryonic stem cell
Molecular biology
Repressor Proteins
cell differentiation
Chromobox Protein Homolog 5
Heterochromatin protein 1
methylation
protein
Carrier Proteins
suppressor of zeste 12 protein
Transcription Factors
Subjects
Details
- ISSN :
- 09673849
- Volume :
- 15
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
- Accession number :
- edsair.doi.dedup.....56568c80800542a6575927e84f0abb90