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Bio-extract amalgamated sodium alginate-cellulose nanofibres based 3D-sponges with interpenetrating BioPU coating as potential wound care scaffolds
- Source :
- Materials Science and Engineering: C. 118:111348
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- In this work, sodium alginate (SA) based “all-natural” composite bio-sponges were designed for potential application as wound care scaffold. The composite bio-sponges were developed from the aqueous amalgamation of SA and cellulose nanofibres (CNFs) in bio-extracts like Rice water (Rw) and Giloy extract (Ge). These sponges were modified by employing a simple coating strategy using vegetable oil-based bio-polyurethane (BioPU) to tailor their physicochemical and biological properties so as to match the specific requirements of a wound care scaffold. Bio-sponges with shared interpenetrating polymeric network structures were attained at optimized BioPU coating formulation. The interpenetration of BioPU chains within the sponge construct resulted in the formation of numerous micro-networks in the interconnected microporous structure of sponges (porosity ≥75%). The coated sponge showed a superior mechanical strength (compressive strength ~3.8 MPa, compressive modulus ~35 MPa) with appreciable flexibility and recoverability under repeated compressive loading-unloading cycles. A tunable degradation behaviour was achieved by varying BioPU coating concentrations owing to the different degree of polymer chain entanglement within the sponge construct. The physical entanglement of BioPU chains with core structural components of sponge improved their structural stability by suppressing their full fragmentation in water-based medium without affecting its swelling behaviour (swelling ratio > 1000%). The coated sponge surface has provided a suitable moist-adherent physical environment to support the adhesion and growth of skin cells (HaCaT cells). The MTT (3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide) assay and hemolytic assay revealed the non-toxic and biocompatible nature of coated sponges in vitro. Moreover, no signs of skin erythema or edema were observed during in vivo dermal irritation and corrosion test performed on the skin of Sprague Dawley (SD) rats. Our initial observations revealed the credibility of these sponges as functional wound care scaffolds as well as its diverse potential as a suitable substrate for various tissue engineering applications.
- Subjects :
- Skin erythema
Materials science
Biocompatibility
Alginates
Polyurethanes
Nanofibers
Bioengineering
02 engineering and technology
engineering.material
010402 general chemistry
01 natural sciences
Rats, Sprague-Dawley
Biomaterials
chemistry.chemical_compound
food
Coating
medicine
Animals
Cellulose
Tissue Engineering
Tissue Scaffolds
biology
Plant Extracts
Rice water
021001 nanoscience & nanotechnology
biology.organism_classification
food.food
Rats
0104 chemical sciences
Sponge
Compressive strength
chemistry
Chemical engineering
Mechanics of Materials
engineering
Swelling
medicine.symptom
0210 nano-technology
Porosity
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 118
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....565527e3f4bb55630aca2b87059bc154