A simple high-performance liquid chromatographic method for estimating human serum angiotensin-converting enzyme activity

Authors :: A. Pitotti
Mariarosa Moneghini
Luciano Vio
V. Maurich
Maurich, V.
Moneghini, Mariarosa
Pitotti, A.
Vio, L.
Source :: Journal of pharmaceutical and biomedical analysis. 3(1)
Publication Year :: 1983
Abstract: A simple method for measuring angiotensin-converting enzyme activity in human serum was developed. Samples were incubated with hippurylhistidylleucine and the liberated hippuric acid was determined directly by reversed phase ion-pair high-performance liquid chromatography with UV spectrometric detection.

Subjects :: Chromatography
biology
Chemistry
Clinical Biochemistry
Serum angiotensin converting enzyme
Pharmaceutical Science
Hippuric acid
Angiotensin-converting enzyme
Reversed-phase chromatography
HPLC
angiotensin-converting enzyme activity
Enzyme assay
Analytical Chemistry
chemistry.chemical_compound
Drug Discovery
biology.protein
Spectroscopy

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