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Dendritic Cells Require the NF-κB2 Pathway for Cross-Presentation of Soluble Antigens
- Source :
- The Journal of Immunology. 181:354-363
- Publication Year :
- 2008
- Publisher :
- The American Association of Immunologists, 2008.
-
Abstract
- NF-κB-inducing kinase (NIK) is responsible for activation of the non-canonical p100 processing pathway of NF-κB activation. This kinase has been shown to be critical for activation of this pathway after signaling through several TNF family members including CD40. The functional importance of this pathway in CD40 and TLR-induced dendritic cell (DC) differentiation was studied in vivo in the alymphoplasia (Aly) mouse. The Aly mouse expresses a mutant NIK molecule that prohibits the induction of the non-canonical pathway. We show that while MHC class II presentation and in vivo migration of Aly DCs is intact, these cells are unable to cross-prime CD8+ T cells to exogenous Ag. Gene expression array analysis of DCs matured in vivo indicates multiple defects in Ag processing pathways after maturation and provide a global view of the genes that are regulated by the NF-κB2 pathway in DCs. These experiments indicate a possible role for NIK in mediating cross-priming of soluble Ag. In addition, our findings explain the profound immune unresponsiveness of the Aly mouse.
- Subjects :
- Immunology
Active Transport, Cell Nucleus
Cell Line
Mice
Cross-Priming
NF-kappa B p52 Subunit
Cell Movement
Animals
Immunology and Allergy
Antigens
Lymphatic Diseases
Regulation of gene expression
MHC class II
CD40
biology
Kinase
Gene Expression Profiling
Cell Membrane
Toll-Like Receptors
Cross-presentation
Dendritic Cells
Dendritic cell
Up-Regulation
Cell biology
Phenotype
Gene Expression Regulation
Solubility
Cell culture
Mutation
biology.protein
CD8
Signal Transduction
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 181
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....562be417eb958a6bed9e5dffd7f7848e