Supplementary Figure 3 from Immune Response Is an Important Aspect of the Antitumor Effect Produced by a CD40L-Encoding Oncolytic Adenovirus

PDF file - 75K, (A) MB49 cells were infected with 100 viral particles/cell with Ad5/3-CMV-mCD40L and Ad5/3-Luc1 and Annexin-V was assessed 24 hours post-infection by flow cytometry. (B) Replication of Ad5/3-CMV-mCD40L and Ad5/3-Luc1 over time in MB49 cell line. Cell monolayers were infected at doses specified ranging from 101 to 108 viral particles and assays were stopped at different time points (days 2, 3, 4 and 8). Cell viability was measured and expressed overtime. (C) MB49 cells were infected with 100 viral particles and samples were collected at 0, 24, 48 and 72 hours post infection. Quantitative PCR was performed for E4 sequence and values were normalized to mouse beta-actin gene.