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Stem cell recovery from cyclophosphamide-induced myelosuppression requires the presence of CD4+cells
- Source :
- British Journal of Haematology. 75:168-174
- Publication Year :
- 1990
- Publisher :
- Wiley, 1990.
-
Abstract
- Summary Recently, we have reviewed studies regarding the growth-stimulating effect of CD4+ cells on haematopoietic cells in culture (Pantel & Nakeff, 1989a). In the present study we have tested the physiologic relevance of this interaction using a drug-perturbed mouse model. The long-term application of cyclophosphamide (CY, 30 mg/kg/d, five i.p. injections per week over 7 weeks) in B6D2F1 mice resulted in initial CY-induced cytotoxicity to CFU-S followed by the re-establishment to pretreatment values of the femoral content of CFU-S within 2–3 weeks of CY-treatment. An examination of CY-metabolism in these treated mice excluded a pharmacological explanation for the compensation of CY-cytotoxicity. However, a three-fold increase in the cycling fraction of CFU-S (determined by in vivo hydroxyurea suicide) was observed concomitant with a two-fold increase in the femoral content of L3T4+ cells (the murine equivalent to human CD4+ cells), as compared to the corresponding values in untreated mice. Ablating these L3T4+ cells in vivo by means of a cytotoxic monoclonal antibody (MoAb) to the L3T4 determinant resulted in a decrease in the cycling fraction of CFU-S from 56.8% to essentially zero and a decrease in the femoral content of CFU-S when comparing mice receiving either CY alone or CY plus MoAb, respectively. It would appear that the CY-induced increase in the proliferative activity of CFU-S requires the presence of L3T4+ cells. These data constitute the first in situ evidence for the physiologic relevance of immunocompetent L3T4+ cells as regulators involved in the recovery of stem cells from drug-induced myelosuppression.
- Subjects :
- Male
Cyclophosphamide
T-Lymphocytes
Mice, Inbred Strains
Cell Communication
Pharmacology
Colony-Forming Units Assay
Mice
In vivo
Animals
Cytotoxic T cell
Medicine
Cytotoxicity
Cells, Cultured
business.industry
Hematology
Hematopoietic Stem Cells
Haematopoiesis
medicine.anatomical_structure
CD4 Antigens
Immunology
Toxicity
Bone marrow
Stem cell
business
Cell Division
medicine.drug
Subjects
Details
- ISSN :
- 13652141 and 00071048
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- British Journal of Haematology
- Accession number :
- edsair.doi.dedup.....56254cbfce469c9aab006d4fed4bbd11