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Metabolome analysis of esophageal cancer tissues using capillary electrophoresis-time-of-flight mass spectrometry
- Source :
- International Journal of Oncology.
- Publication Year :
- 2018
- Publisher :
- Spandidos Publications, 2018.
-
Abstract
- Reports of the metabolomic characteristics of esophageal cancer are limited. In the present study, we thus conducted metabolome analysis of paired tumor tissues (Ts) and non-tumor esophageal tissues (NTs) using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). The Ts and surrounding NTs were surgically excised pair-wise from 35 patients with esophageal cancer. Following tissue homogenization and metabolite extraction, a total of 110 compounds were absolutely quantified by CE-TOFMS. We compared the concentrations of the metabolites between Ts and NTs, between pT1 or pT2 (pT1-2) and pT3 or pT4 (pT3-4) stage, and between node-negative (pN-) and node-positive (pN+) samples. Principal component analysis and hierarchical clustering analysis revealed clear metabolomic differences between Ts and NTs. Lactate and citrate levels in Ts were significantly higher (P=0.001) and lower (P
- Subjects :
- Adult
Male
0301 basic medicine
Cancer Research
Esophageal Neoplasms
Metabolite
Biology
Mass Spectrometry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Metabolomics
Metabolome
Humans
Prospective Studies
Precision Medicine
Aged
Neoplasm Staging
Aged, 80 and over
chemistry.chemical_classification
Principal Component Analysis
Glutaminolysis
Electrophoresis, Capillary
Tricarboxylic acid
Middle Aged
Molecular biology
Glutamine
030104 developmental biology
Oncology
chemistry
Tumor progression
030220 oncology & carcinogenesis
Female
Malic acid
Subjects
Details
- ISSN :
- 17912423 and 10196439
- Database :
- OpenAIRE
- Journal :
- International Journal of Oncology
- Accession number :
- edsair.doi.dedup.....5613cff74fb82477f4c3ab64c9b2989f
- Full Text :
- https://doi.org/10.3892/ijo.2018.4340