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Sorbus alnifolia protects dopaminergic neurodegeneration in Caenorhabditis elegans
- Source :
- Pharmaceutical biology, Pharmaceutical Biology, Vol 55, Iss 1, Pp 481-486 (2017)
- Publication Year :
- 2016
-
Abstract
- Context: The twigs of Sorbus alnifolia (Sieb. et Zucc.) K. Koch (Rosaceae) have been used to treat neurological disorders as a traditional medicine in Korea. However, there are limited data describing the efficacy of S. alnifolia in Parkinson’s disease (PD). Objective: This study was conducted to identify the protective effects of the methanol extracts of S. alnifolia (MESA) on the dopaminergic (DA) neurodegeneration in Caenorhabditis elegans. Materials and methods: To test the neuroprotective action of MESA, viability assay was performed after 48 h exposure to 1-methyl-4-phenylpyridine (MMP+) in PC12 cells and C. elegans (400 μM and 2 mM of MMP+, respectively). Fluorescence intensity was quantified using transgenic mutants such as BZ555 (Pdat-1::GFP) and and UA57 (Pdat-1::GFP and Pdat-1::CAT-2) to determine MESA’s effects on DA neurodegeneration in C. elegans. Aggregation of α-synuclein was observed using NL5901 strain (unc-54p::α-synuclein::YFP). MESA’s protective effects on the DA neuronal functions were examined by food-sensing assay. Lifespan assay was conducted to test the effects of MESA on the longevity. Results: MESA restored MPP+-induced loss of viability in both PC12 cells and C. elegans (85.8% and 54.9%, respectively). In C. elegans, MESA provided protection against chemically and genetically-induced DA neurodegeneration, respectively. Moreover, food-sensing functions were increased 58.4% by MESA in the DA neuron degraded worms. MESA also prolonged the average lifespan by 25.6%. However, MESA failed to alter α-synuclein aggregation. Discussion and conclusions: These results revealed that MESA protects DA neurodegeneration and recovers diminished DA neuronal functions, thereby can be a valuable candidate for the treatment of PD.
- Subjects :
- 0301 basic medicine
1-Methyl-4-phenylpyridinium
Time Factors
Pharmaceutical Science
Pharmacology
PC12 Cells
Green fluorescent protein
Animals, Genetically Modified
lifespan-extension
tyrosine hydroxylase
Drug Discovery
neurotoxicity
Sorbus
Caenorhabditis elegans
biology
Behavior, Animal
Sorbus alnifolia
Dopaminergic
Neurodegeneration
General Medicine
Caenorhabditis
Neuroprotective Agents
food sensing
alpha-Synuclein
Molecular Medicine
Cell Survival
Longevity
Motor Activity
Neuroprotection
Article
03 medical and health sciences
Protein Aggregates
Botany
medicine
Animals
Viability assay
cardiovascular diseases
Caenorhabditis elegans Proteins
Plants, Medicinal
Dose-Response Relationship, Drug
Plant Extracts
Dopaminergic Neurons
Methanol
lcsh:RM1-950
nutritional and metabolic diseases
Feeding Behavior
Plant Components, Aerial
biology.organism_classification
medicine.disease
Rats
030104 developmental biology
lcsh:Therapeutics. Pharmacology
Complementary and alternative medicine
Cytoprotection
Nerve Degeneration
Solvents
Parkinson’s disease
Phytotherapy
Subjects
Details
- ISSN :
- 17445116
- Volume :
- 55
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical biology
- Accession number :
- edsair.doi.dedup.....5600edbd33d84ef23d5497099e68bd51