Back to Search
Start Over
Isolation and characterization of cluster of differentiation 9-positive ependymal cells as potential adult neural stem/progenitor cells in the third ventricle of adult rats
- Source :
- Cell and tissue research. 379(3)
- Publication Year :
- 2019
-
Abstract
- Ependymal cells located above the ventricular zone of the lateral, third, and fourth ventricles and the spinal cord are thought to form part of the adult neurogenic niche. Many studies have focused on ependymal cells as potential adult neural stem/progenitor cells. To investigate the functions of ependymal cells, a simple method to isolate subtypes is needed. Accordingly, in this study, we evaluated the expression of cluster of differentiation (CD) 9 in ependymal cells by in situ hybridization and immunohistochemistry. Our results showed that CD9-positive ependymal cells were also immunopositive for SRY-box 2, a stem/progenitor cell marker. We then isolated CD9-positive ependymal cells from the third ventricle using the pluriBead-cascade cell isolation system based on antibody-mediated binding of cells to beads of different sizes and their isolation with sieves of different mesh sizes. As a result, we succeeded in isolating CD9-positive populations with 86% purity of ependymal cells from the third ventricle. We next assayed whether isolated CD9-positive ependymal cells had neurospherogenic potential. Neurospheres were generated from CD9-positive ependymal cells of adult rats and were immunopositve for neuron, astrocyte, and oligodendrocyte markers after cultivation. Thus, based on these findings, we suggest that the isolated CD9-positive ependymal cells from the third ventricle included tanycytes, which are special ependymal cells in the ventricular zone of the third ventricle that form part of the adult neurogenic and gliogenic niche. These current findings improve our understanding of tanycytes in the adult third ventricle in vitro.
- Subjects :
- 0301 basic medicine
Male
Histology
Ependymal Cell
Biology
Tetraspanin 29
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Neural Stem Cells
Neurosphere
Ependyma
medicine
Animals
Progenitor cell
Rats, Wistar
In Situ Hybridization
Cell Proliferation
Third Ventricle
Third ventricle
Cluster of differentiation
Stem Cells
Cell Differentiation
Cell Biology
Immunohistochemistry
Oligodendrocyte
Cell biology
Rats
030104 developmental biology
medicine.anatomical_structure
Neuron
030217 neurology & neurosurgery
Astrocyte
Subjects
Details
- ISSN :
- 14320878
- Volume :
- 379
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cell and tissue research
- Accession number :
- edsair.doi.dedup.....55e731f5e734e5379b806131dcdd11ac