Back to Search
Start Over
Serotonin Promotes Serum Albumin Interaction with the Monomeric Amyloid β Peptide
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 5896, p 5896 (2021), International Journal of Molecular Sciences, Volume 22, Issue 11
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Prevention of amyloid β peptide (Aβ) deposition via facilitation of Aβ binding to its natural depot, human serum albumin (HSA), is a promising approach to preclude Alzheimer’s disease (AD) onset and progression. Previously, we demonstrated the ability of natural HSA ligands, fatty acids, to improve the affinity of this protein to monomeric Aβ by a factor of 3 (BBRC, 510(2), 248–253). Using plasmon resonance spectroscopy, we show here that another HSA ligand related to AD pathogenesis, serotonin (SRO), increases the affinity of the Aβ monomer to HSA by a factor of 7/17 for Aβ40/Aβ42, respectively. Meanwhile, the structurally homologous SRO precursor, tryptophan (TRP), does not affect HSA’s affinity to monomeric Aβ, despite slowdown of the association and dissociation processes. Crosslinking with glutaraldehyde and dynamic light scattering experiments reveal that, compared with the TRP-induced effects, SRO binding causes more marked changes in the quaternary structure of HSA. Furthermore, molecular docking reveals distinct structural differences between SRO/TRP complexes with HSA. The disintegration of the serotonergic system during AD pathogenesis may contribute to Aβ release from HSA in the central nervous system due to impairment of the SRO-mediated Aβ trapping by HSA.
- Subjects :
- Molecular Conformation
Ligands
Surface plasmon resonance
Biology (General)
Spectroscopy
amyloid β peptide
biology
Chemistry
intrinsic disorder
Temperature
General Medicine
Human serum albumin
Computer Science Applications
serotonin
Molecular Docking Simulation
human serum albumin
embryonic structures
Alzheimer’s disease
surface plasmon resonance
Protein Binding
medicine.drug
QH301-705.5
Serum albumin
Serum Albumin, Human
Molecular Dynamics Simulation
Serotonergic
Article
Catalysis
Inorganic Chemistry
Structure-Activity Relationship
Alzheimer Disease
medicine
Humans
tryptophan
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Amyloid beta-Peptides
Binding Sites
Ligand
Organic Chemistry
Tryptophan
molecular docking
body regions
biology.protein
Biophysics
Protein quaternary structure
Serotonin
Protein Multimerization
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 5896
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....55a7cb62cbd3fcb3f1a9fc9c58b014a5