Back to Search Start Over

Serotonin Promotes Serum Albumin Interaction with the Monomeric Amyloid β Peptide

Authors :
Elena V Raznikova
Aliya A. Nazipova
Marina P. Shevelyova
Maria E. Permyakova
Ekaterina A. Litus
Sergei E. Permyakov
Evgenia I. Deryusheva
Ekaterina L. Nemashkalova
Vladimir N. Uversky
Alexey S. Kazakov
Source :
International Journal of Molecular Sciences, Vol 22, Iss 5896, p 5896 (2021), International Journal of Molecular Sciences, Volume 22, Issue 11
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Prevention of amyloid β peptide (Aβ) deposition via facilitation of Aβ binding to its natural depot, human serum albumin (HSA), is a promising approach to preclude Alzheimer’s disease (AD) onset and progression. Previously, we demonstrated the ability of natural HSA ligands, fatty acids, to improve the affinity of this protein to monomeric Aβ by a factor of 3 (BBRC, 510(2), 248–253). Using plasmon resonance spectroscopy, we show here that another HSA ligand related to AD pathogenesis, serotonin (SRO), increases the affinity of the Aβ monomer to HSA by a factor of 7/17 for Aβ40/Aβ42, respectively. Meanwhile, the structurally homologous SRO precursor, tryptophan (TRP), does not affect HSA’s affinity to monomeric Aβ, despite slowdown of the association and dissociation processes. Crosslinking with glutaraldehyde and dynamic light scattering experiments reveal that, compared with the TRP-induced effects, SRO binding causes more marked changes in the quaternary structure of HSA. Furthermore, molecular docking reveals distinct structural differences between SRO/TRP complexes with HSA. The disintegration of the serotonergic system during AD pathogenesis may contribute to Aβ release from HSA in the central nervous system due to impairment of the SRO-mediated Aβ trapping by HSA.

Details

Language :
English
ISSN :
16616596 and 14220067
Volume :
22
Issue :
5896
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences
Accession number :
edsair.doi.dedup.....55a7cb62cbd3fcb3f1a9fc9c58b014a5