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Dynamic changes in the TRPA1 selectivity filter lead to progressive but reversible pore dilation
- Source :
- Banke, T G, Chaplan, S R & Wickenden, A D 2010, ' Dynamic changes in the TRPA1 selectivity filter lead to progressive but reversible pore dilation ' A J P: Cell Physiology (Online), vol. 298, no. 6, pp. C1457-68 . https://doi.org/10.1152/ajpcell.00489.2009
- Publication Year :
- 2010
-
Abstract
- TRPA1 is a nonselective cation channel belonging to the transient receptor potential (TRP) family that is expressed in peripheral sensory neurons and may play important roles in pain perception and inflammation. We found that agonist stimulation of TRPA1, along with other members of the TRP family (TRPV1–4 and TRPM8), can induce the appearance of a large pore permeable to large organic cations such as Yo-Pro (YP) and N-methyl-d-glucamine, in an agonist and divalent cation-dependent manner. YP uptake was not inhibited by a panel of putative gap junction/pannexin blockers, suggesting that gap junction proteins are not required in this process. Our data suggest that changes in the TRP channel selectivity filter itself result in a progressive but reversible pore dilation process, a process that is under strong regulation by external calcium ions. Our data suggest that calcium plays a novel role in setting the amount of time TRPA1 channels spend in a dilated state providing a mechanism that may limit sensory neuron activation by painful or irritating substances.
- Subjects :
- Agonist
Purinergic P2 Receptor Agonists
Cell Membrane Permeability
Physiology
medicine.drug_class
TRPM Cation Channels
TRPV Cation Channels
Nerve Tissue Proteins
CHO Cells
Transfection
Transient receptor potential channel
Cricetulus
Dogs
Meglumine
Transient Receptor Potential Channels
Isothiocyanates
Cricetinae
Membrane Transport Modulators
medicine
Animals
Humans
TRPA1 Cation Channel
Benzoxazoles
Voltage-dependent calcium channel
Dose-Response Relationship, Drug
Chemistry
Receptors, Purinergic P2
Quinolinium Compounds
Cell Membrane
Gap junction
Cell Biology
Pannexin
Farnesol
Sensory neuron
Salicylates
Rats
Kinetics
medicine.anatomical_structure
Biochemistry
Benzamides
Biophysics
Calcium
Calcium Channels
Carbamates
Receptors, Purinergic P2X7
Ion Channel Gating
Subjects
Details
- ISSN :
- 15221563
- Volume :
- 298
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- American journal of physiology. Cell physiology
- Accession number :
- edsair.doi.dedup.....55a497b33787de51a6fbe824255e342e