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Controlling burst effect with PLA/PVA coaxial electrospun scaffolds loaded with BMP-2 for bone guided regeneration
- Source :
- Materials Science and Engineering: C. 97:602-612
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Biocompatible scaffolds have been used to promote cellular growth and proliferation in order to develop grafts, prostheses, artificial skins and cartilage. Electrospinning is widely studied as a method capable of producing nanofibers which enables cell attachment and proliferation, generating a functional scaffold that is suitable for many types of organs or tissues. In this study, electrospinning was used to obtain core-shell and monolithic fibers from the biocompatible poly (lactic acid) and poly (vinyl alcohol) polymers. The main purpose of this work is to produce core-shell nanofiber based scaffolds that works as a sustained delivery vehicle for BMP-2 protein, allowing those fibers to be used in the recovery of alveolar bone tissue without further bone surgery. Then, polymer nanofibers were manufactured by optimizing process parameters of coaxial electrospinning with emphasis on the most relevant ones: voltage, internal and external flows in an attempt to correlate fibers properties with protein releasing abilities. All nanofibers were characterized according to its morphology, thermal behaviour, crystallinity and release profile. For the release tests, bovine albumin was added into internal fiber for future periodontal restorage application. Obtained results demonstrate that fibers were formed with diameters up to 250 nm. According to electronic microscopy images, one could observe surface of nanofibers, thickness and core-shell morphology confirmed. X-ray diffraction analysis and contact angle tests showed fibers with low crystal degree and low hydrophobicity. Nanofibers structure affected in vitro release model tests and consequently the cellular assays.
- Subjects :
- Vinyl alcohol
Scaffold
Materials science
Cell Survival
Polyesters
Nanofibers
Bone Morphogenetic Protein 2
Biocompatible Materials
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Bone and Bones
Cell Line
Biomaterials
Contact angle
Mice
chemistry.chemical_compound
Tissue engineering
Transforming Growth Factor beta
Animals
Humans
Regeneration
Fiber
Cell Proliferation
chemistry.chemical_classification
Drug Carriers
Tissue Engineering
Cell Differentiation
Polymer
021001 nanoscience & nanotechnology
Recombinant Proteins
Electrospinning
0104 chemical sciences
chemistry
Mechanics of Materials
Polyvinyl Alcohol
Nanofiber
0210 nano-technology
Biomedical engineering
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....55a1ab9483c30862f0b15c83ec8c800d