Back to Search
Start Over
CircRNA circ_0008037 facilitates tumor growth and the Warburg effect via upregulating NUCKS1 by binding to miR‐433‐3p in non‐small cell lung cancer
- Source :
- Thoracic Cancer, Vol 13, Iss 2, Pp 162-172 (2022), Thoracic Cancer
- Publication Year :
- 2022
- Publisher :
- Wiley, 2022.
-
Abstract
- Background Circular RNAs (circRNAs) participate in the genesis and progression of tumors, including non‐small cell lung cancer (NSCLC). At present, the role and regulatory mechanisms of circRNAs in NSCLC have not been fully elucidated. The aim of this study was to explore the role and regulatory mechanism of circRNA hsa_circ_0008037 (circ_0008037) in NSCLC. Methods Expression of circ_0008037 in NSCLC tissues and cells was detected by quantitative real‐time polymerase chain reaction (RT‐qPCR). Loss‐of‐function experiments were performed to analyze the influence of circ_0008037 knockdown on proliferation, migration, invasion, and the Warburg effect of NSCLC cells. Western blotting was utilized for protein analysis. The regulatory mechanism of circ_0008037 was surveyed by bioinformatics analysis, RNA pulldown assay, and dual‐luciferase reporter assay. Xenograft assay was used to validate the oncogenicity of circ_0008037 in NSCLC in vivo. Results Circ_0008037 was upregulated in NSCLC tissues and cells. Circ_0008037 downregulation reduced tumor growth in vivo and repressed proliferation, migration, invasion, and decreased the Warburg effect of NSCLC cells in vitro. Mechanically, circ_0008037 regulated nuclear ubiquitous casein kinase and cyclin‐dependent kinase substrate 1 (NUCKS1) expression via sponging miR‐433‐3p. Furthermore, MiR‐433‐3p inhibitor reversed the inhibiting influence of circ_0008037 silencing on proliferation, migration, invasion, and the Warburg effect of NSCLC cells. Also, NUCKS1 elevation overturned the repressive influence of miR‐433‐3p mimic on proliferation, migration, invasion, and the Warburg effect of NSCLC cells. Conclusion Circ_0008037 accelerated tumor growth and elevated the Warburg effect via regulating NUCKS1 expression by adsorbing miR‐433‐3p, providing an underlying target for NSCLC treatment.<br />A model depicting the regulatory mechanism of circ_0008037 in NSCLC. Circ_0008037 elevates NUCKS1 expression through binding to miR‐433‐3p and inhibiting miR‐433‐3p activity, promoting NSCLC tumorigenesis.
- Subjects :
- Male
Pulmonary and Respiratory Medicine
Lung Neoplasms
NUCKS1
NSCLC
circ_0008037
Mice
Downregulation and upregulation
Cell Movement
Carcinoma, Non-Small-Cell Lung
Animals
Humans
Medicine
Gene silencing
Lung cancer
RC254-282
Cell Proliferation
Mice, Inbred BALB C
Gene knockdown
Reporter gene
business.industry
miR‐433‐3p
Nuclear Proteins
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RNA, Circular
Original Articles
General Medicine
Middle Aged
Phosphoproteins
medicine.disease
Warburg effect
Up-Regulation
respiratory tract diseases
Blot
MicroRNAs
Oncology
A549 Cells
Cancer research
Female
Original Article
Casein kinase 1
business
Subjects
Details
- Language :
- English
- ISSN :
- 17597706 and 17597714
- Volume :
- 13
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Thoracic Cancer
- Accession number :
- edsair.doi.dedup.....558213e12521338211f2a314ae4b27dc