Back to Search
Start Over
Graphene oxide as a nanocarrier for loading and delivery of medicinal drugs and as a biosensor for detection of serum albumin
- Source :
- Analytica Chimica Acta. 769:40-48
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- The interaction of graphene oxide (GO), a medicinal drug (10-hydroxy camptothecin (HCPT)), and bovine serum albumin (BSA) was investigated with the aim of developing a method for the analysis of serum albumin proteins. It was demonstrated that HCPT could be readily loaded onto GO via the π–π stacking interaction, and the delivery of HCPT to BSA was improved in the presence of GO; this, in turn, facilitated the binding interaction of HCPT and BSA. Chemometrics methods, multivariate curve resolution-alternating least squares (MCR-ALS) and parallel factor analysis (PARAFAC), were applied to resolve spectral data, and this assisted in the elucidation of the above interaction. GO was found to enhance the fluorescence response of HCPT to BSA, and thus, a low cost fluorescence bio-sensing platform was developed for fluorescence-enhanced detection of BSA based on GO. The satisfactory analytical performance of this biosensor for BSA was attributed to the structure and electronic properties of GO.
- Subjects :
- Serum albumin
Biosensing Techniques
Biochemistry
Analytical Chemistry
law.invention
Chemometrics
law
Fluorescence Resonance Energy Transfer
medicine
Environmental Chemistry
Least-Squares Analysis
Bovine serum albumin
Spectroscopy
Drug Carriers
Chromatography
biology
Chemistry
Graphene
Oxides
Serum Albumin, Bovine
Fluorescence
Nanostructures
biology.protein
Camptothecin
Graphite
Nanocarriers
Biosensor
Protein Binding
medicine.drug
Subjects
Details
- ISSN :
- 00032670
- Volume :
- 769
- Database :
- OpenAIRE
- Journal :
- Analytica Chimica Acta
- Accession number :
- edsair.doi.dedup.....557d482dc6439b3d28ce9845d4eb99c9