Back to Search
Start Over
Lipid Droplets Accumulate in the Hypothalamus of Mice and Humans with and without Metabolic Diseases
- Source :
- Neuroendocrinology, 111, 263-272. S. Karger AG, Neuroendocrinology 111, 263-272 (2021), Neuroendocrinology, 111(3), 263-272. Karger, Neuroendocrinology, 111(3), 263-272. S. Karger AG
- Publication Year :
- 2021
-
Abstract
- Background: In peripheral tissues, the lipid droplet (LD) organelle links lipid metabolism, inflammation, and insulin resistance. Little is known about the brain LDs. Objectives: We hypothesized that hypothalamic LDs would be altered in metabolic diseases. Methods: We used immunofluorescence labeling of the specific LD protein, PLIN2, as the approach to visualize and quantify LDs. Results: LDs were abundant in the hypothalamic third ventricle wall layer with similar heterogeneous distributions between control mice and humans. The LD content was enhanced by high-fat diet (HFD) in both wild-type and in low-density lipoprotein receptor deficient (Ldlr –/– HFD) mice. Strikingly, we observed a lower LD amount in type 2 diabetes mellitus (T2DM) patients when compared with non-T2DM patients. Conclusions: LDs accumulate in the normal hypothalamus, with similar distributions in human and mouse. Moreover, metabolic diseases differently modify LD content in mouse and human. Our results suggest that hypothalamic LD accumulation is an important target to the study of metabolism.
- Subjects :
- Male
medicine.medical_specialty
type 2 diabetes mellitus
Endocrinology, Diabetes and Metabolism
Hypothalamus
lipid droplet
Inflammation
Tissue Banks
Diet, High-Fat
Immunofluorescence
Perilipin-2
Mice
Cellular and Molecular Neuroscience
Endocrinology
Insulin resistance
Internal medicine
Lipid droplet
insulin resistance
Organelle
medicine
Animals
Humans
Aged
Aged, 80 and over
Mice, Knockout
medicine.diagnostic_test
Endocrine and Autonomic Systems
Chemistry
Type 2 Diabetes Mellitus
Lipid metabolism
Lipid Droplets
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
PLIN2
Diabetes Mellitus, Type 2
Receptors, LDL
Insulin Resistance
Lipid Droplet
Plin2
Female
Autopsy
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 00283835
- Volume :
- 111
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Neuroendocrinology
- Accession number :
- edsair.doi.dedup.....557122f6d0a193176d37459231297748