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Cone‐setting in spruce is regulated by conserved elements of the age‐dependent flowering pathway
- Source :
- New Phytologist. 236:1951-1963
- Publication Year :
- 2022
- Publisher :
- Wiley, 2022.
-
Abstract
- Reproductive phase change is well characterized in angiosperm model species, but less studied in gymnosperms. We utilize the early cone-setting acrocona mutant to study reproductive phase change in the conifer Picea abies (Norway spruce), a gymnosperm. The acrocona mutant frequently initiates cone-like structures, called transition shoots, in positions where wild-type P. abies always produces vegetative shoots. We collect acrocona and wild-type samples, and RNA-sequence their messenger RNA (mRNA) and microRNA (miRNA) fractions. We establish gene expression patterns and then use allele-specific transcript assembly to identify mutations in acrocona. We genotype a segregating population of inbred acrocona trees. A member of the SQUAMOSA BINDING PROTEIN-LIKE (SPL) gene family, PaSPL1, is active in reproductive meristems, whereas two putative negative regulators of PaSPL1, miRNA156 and the conifer specific miRNA529, are upregulated in vegetative and transition shoot meristems. We identify a mutation in a putative miRNA156/529 binding site of the acrocona PaSPL1 allele and show that the mutation renders the acrocona allele tolerant to these miRNAs. We show co-segregation between the early cone-setting phenotype and trees homozygous for the acrocona mutation. In conclusion, we demonstrate evolutionary conservation of the age-dependent flowering pathway and involvement of this pathway in regulating reproductive phase change in the conifer P. abies.
- Subjects :
- flowering
Evolutionary Biology
Picea abies
Physiology
Reproduction
Forest Science
Meristem
Plant Science
reproductive development
Evolutionsbiologi
Tracheophyta
gymnosperm
Gene Expression Regulation, Plant
SPL-gene family
Plant Biotechnology
Picea
transcriptome
cone-setting
Växtbioteknologi
Plant Proteins
Subjects
Details
- ISSN :
- 14698137 and 0028646X
- Volume :
- 236
- Database :
- OpenAIRE
- Journal :
- New Phytologist
- Accession number :
- edsair.doi.dedup.....5555c4573432355e46d8866ddd2ab53b
- Full Text :
- https://doi.org/10.1111/nph.18449