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Distribution of Gs-? activating mutations in human thyroid tumors measured by subcloning
- Source :
- Journal of Cancer Research and Clinical Oncology. 122:453-457
- Publication Year :
- 1996
- Publisher :
- Springer Science and Business Media LLC, 1996.
-
Abstract
- In the search for the prevalence and distribution pattern of Gs-alpha gene mutations in differentiated thyroid tumors we examined 66 tumor tissue samples for the presence of mutations at "hot-spot" codons 201 and 227 using methods based on the polymerase chain reaction, subcloning and sequencing. The prevailing type of single-base substitution at codon 201 (71.4%) corresponded to the replacement of the wild-type sequence CGT (Arg) with TGT (Cys). The fragments of the Gs-alpha gene, including codon 201 or 227 from five follicular carcinomas and one follicular adenoma, were subcloned in Escherichia coli and it was found that the proportion of alleles with mutated codon 201 varied from 3.2% to 43%. Sequencing of the corresponding region has confirmed preliminary data indicating that the single-base changes CGT (Arg) to TGT (Cys) or CGT to CAT (His) occurred. There was only a weak correlation between the prevalence of cells bearing a mutation in the Gs-alpha gene and the level of Gs-alpha protein expression in the corresponding thyroid tumors.
- Subjects :
- Adult
Male
Cancer Research
Molecular Sequence Data
Biology
Molecular cloning
Gene mutation
medicine.disease_cause
law.invention
GTP-Binding Proteins
law
Escherichia coli
medicine
Humans
Thyroid Neoplasms
Cloning, Molecular
Codon
Gene
Polymerase chain reaction
Aged
Genetics
Mutation
Base Sequence
Thyroid
Nucleic acid sequence
DNA
General Medicine
Middle Aged
Molecular biology
Subcloning
medicine.anatomical_structure
Oncology
Female
Subjects
Details
- ISSN :
- 14321335 and 01715216
- Volume :
- 122
- Database :
- OpenAIRE
- Journal :
- Journal of Cancer Research and Clinical Oncology
- Accession number :
- edsair.doi.dedup.....5554536595bd95dced1f6104ec70f69f