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Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani
- Source :
- World journal of microbiologybiotechnology. 31(1)
- Publication Year :
- 2014
-
Abstract
- Several volatile organic compounds (VOCs) producing endophyte bacteria were isolated from the leaves of olive trees and tested for their antifungal activity against several pathogenic fungi. An antagonistic strain called P2 showed 97 % of homology with Pseudomonas sp. strains on the basis of its 16S rDNA sequence and biochemical properties. P2 strain drastically inhibited the growth of Rhizoctonia solani mycelia (86 %) at 5 day-post-confrontation (dpc) and strongly reduced fungi infection on potato slices at 10(7) bacteria ml(-1) for 3 and 7 dpc. P2 strain was also positive for protease activity as well as siderophore production. Light microscopy analysis showed that treatment of R. solani mycelia with P2 strain induced thickening of the cell-wall, vesiculation of protoplasm and blockage of fungal hyphae branching. VOCs analysis using GC-MS allowed the detection of two major products with m/z of 93.9910 and 125.9630 corresponding to dimethyl disulfide and dimethyl trisulfide respectively. VOCs-producing P2 strain could be a promising agent in the protection of tuber crops against fungal diseases.
- Subjects :
- DNA, Bacterial
Siderophore
Antifungal Agents
Physiology
Molecular Sequence Data
Applied Microbiology and Biotechnology
Endophyte
DNA, Ribosomal
Microbiology
Rhizoctonia
Rhizoctonia solani
chemistry.chemical_compound
Pseudomonas
RNA, Ribosomal, 16S
Cluster Analysis
Dimethyl disulfide
Mycelium
Phylogeny
Base Composition
Microscopy
Volatile Organic Compounds
biology
fungi
food and beverages
General Medicine
Sequence Analysis, DNA
biology.organism_classification
Olive trees
Bacterial Typing Techniques
chemistry
Dimethyl trisulfide
Biotechnology
Subjects
Details
- ISSN :
- 15730972
- Volume :
- 31
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- World journal of microbiologybiotechnology
- Accession number :
- edsair.doi.dedup.....5533ad9375a771408be5c7349cd4ce5e