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Replacement of the Replication Factors of Porcine Circovirus (PCV) Type 2 with Those of PCV Type 1 Greatly Enhances Viral Replication I n V itro
- Source :
- Journal of Virology. 84:8986-8989
- Publication Year :
- 2010
- Publisher :
- American Society for Microbiology, 2010.
-
Abstract
- Porcine circovirus type 1 (PCV1), originally isolated as a contaminant of PK-15 cells, is nonpathogenic, whereas porcine circovirus type 2 (PCV2) causes an economically important disease in pigs. To determine the factors affecting virus replication, we constructed chimeric viruses by swapping open reading frame 1 (ORF1) ( rep ) or the origin of replication (Ori) between PCV1 and PCV2 and compared the replication efficiencies of the chimeric viruses in PK-15 cells. The results showed that the replication factors of PCV1 and PCV2 are fully exchangeable and, most importantly, that both the Ori and rep of PCV1 enhance the virus replication efficiencies of the chimeric viruses with the PCV2 backbone.
- Subjects :
- Circovirus
DNA Replication
Swine
animal diseases
viruses
Immunology
Replication Origin
Virus Replication
Origin of replication
Microbiology
Virus
Cell Line
Open Reading Frames
Viral Proteins
Virology
Animals
biology
DNA replication
virus diseases
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Genome Replication and Regulation of Viral Gene Expression
Porcine circovirus
Viral replication
Cell culture
Insect Science
Circoviridae
Genetic Engineering
Subjects
Details
- ISSN :
- 10985514 and 0022538X
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Journal of Virology
- Accession number :
- edsair.doi.dedup.....5527e00801095179a9c7d3957f4f3eed