Antimüllerian hormone and F2-isoprostanes in the Coronary Artery Risk Development in Young Adults (CARDIA) Study

Objective To examine whether F2-isoprostanes, a marker of systematic oxidative stress, are associated with antimullerian hormone (AMH), an indicator of ovarian reserve, in a population-based cohort of women of black and white ethnicities. Design Cross-sectional analysis. Setting Not applicable. Patients The CARDIA Women’s Study, a population-based cohort. Black (n = 398) and white (n = 432) late reproductive−aged women (mean age 40 ± 3.6 years) without histories of gynecologic surgery. Main Outcome Measures Log-transformed serum AMH concentrations. Results Linear regression models evaluated whether plasma F2-isoprostanes were associated with log-transformed AMH after adjustment for age, race, smoking, body mass index, and oral contraceptive pill use. Higher levels of F2-isoprostanes were associated with lower AMH levels (β −0.048 per standard deviation, 95% confidence interval −0.087, −0.01). The observed associations were stronger at younger ages (P=.04 for interaction between levels of age and F2-isoprostanes). Indicators of other steps in the oxidative stress pathway (superoxide dismutase, paraoxonase activity, oxidized low-density lipoprotein cholesterol, and carotenoids) were not associated with AMH, although lower phospholipase A2 activity (β 0.036 per standard deviation, 95% confidence interval 0.001, 0.071) was associated with lower AMH across all ages. Conclusion In a population-based cohort, higher levels of F2-isoprostanes were associated with lower ovarian reserve, particularly at younger ages.