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Characterization of a monoclonal anti-capsid antibody that cross-reacts with three major primate lentivirus lineages
- Source :
- Virology. 422:402-412
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Mouse monoclonal antibodies with varying specificities against the Gag capsid of simian and human immunodeficiency virus (SIV/HIV) were generated by immunizing mice with whole inactivated SIVagmTYO-1. Monoclonal antibody AG3.0 showed the broadest reactivity recognizing the Gag capsid protein (p24–27) and Gag precursors p38, p55, and p150 of HIV-1, HIV-2, SIVmac, and SIVagm. Using overlapping peptides, the AG3.0 epitope was mapped in capsid to a sequence (SPRTLNA) conserved among HIV-1, HIV-2, SIVrcm, SIVsm/mac, and SIVagm related viruses. Because of its broad cross-reactivity, AG3.0 was used to develop an antigen capture assay with a lower detection limit of 100pg/ml HIV-1 Gag p24. Interestingly, AG3.0 was found to have a faster binding on/off rate for SIVagmVer and SIVmac Gag than for SIVagmSab Gag, possibly due to differences outside the SPRTLNA motif. In addition, the ribonucleic acid (RNA) coding for AG3.0 was sequenced to facilitate the development of humanized monoclonal antibodies.
- Subjects :
- CD4-Positive T-Lymphocytes
medicine.drug_class
AG3.0
animal diseases
viruses
Molecular Sequence Data
Enzyme-Linked Immunosorbent Assay
Cross Reactions
Biology
medicine.disease_cause
Monoclonal antibody
Article
Epitope
Cell Line
Mice
Capsid
Virology
medicine
Animals
Humans
Amino Acid Sequence
Gag
Human immunodeficiency virus
Simian immunodeficiency virus
Antibodies, Monoclonal
virus diseases
RNA
p27
p24
Molecular biology
Epitope mapping
HIV-2
Monoclonal
HIV-1
biology.protein
Capsid Proteins
Antibody
Epitope Mapping
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 422
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....55199a46ad5a54a17f08e6a7499fef28