Back to Search
Start Over
Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma
- Source :
- BMC Genomics, Vol 22, Iss 1, Pp 1-14 (2021), BMC Genomics
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Background Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer. Approximately 80% of patients initially diagnosed with locally advanced or metastatic disease survive only 4–11 months after diagnosis. Tremendous efforts have been made toward understanding the biology of PDAC. Results In this study, we first utilized next-generation sequencing technique and existing microarray datasets to identify significant differentially expressed genes between PDAC and non-tumor adjacent tissue. By comparing top significant survival genes in PDAC Gene Expression Profiling Interactive Analysis database and PDAC transcriptome data from patients, our integrated analysis discovered five potential central genes (i.e., MYEOV, KCNN4, FAM83A, S100A16, and DDX60L). Subsequently, we analyzed the cellular functions of the potential novel oncogenes MYEOV and DDX60L, which are highly expressed in PDAC cells. Notably, the knockdown of MYEOV and DDX60L significantly inhibited the metastasis of cancer cells and induced apoptosis. Further RNA sequencing analyses showed that massive signaling pathways, particularly the TNF signaling pathway and nuclear factor-kappa B (NF-κB) signaling pathway, were affected in siRNA-treated cancer cells. The siDDX60L and siMYEOV significantly inhibited the expression of chemokine CXCL2, which may potentially affect the tumor microenvironment in PDAC tissues. Conclusions The present findings identified the novel oncogene DDX60L, which was highly expressed in PDAC. Transcriptome profiling through siRNA knockdown of DDX60L uncovered its functional roles in the PDAC in humans.
- Subjects :
- endocrine system diseases
MYEOV
DDX60L
Pancreatic ductal adenocarcinoma (PDAC)
Biology
QH426-470
Metastasis
Transcriptome
Cell Line, Tumor
Biomarkers, Tumor
Tumor Microenvironment
medicine
Genetics
Humans
Oncogene
Tumor microenvironment
Gene knockdown
Research
Cancer
Oncogenes
medicine.disease
CXCL2
digestive system diseases
Neoplasm Proteins
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms
Gene expression profiling
Cancer cell
Cancer research
TP248.13-248.65
Carcinoma, Pancreatic Ductal
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 22
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....54df160f70a9273a9786b5c5465e060a