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Use of 13 C Nuclear Magnetic Resonance To Assess Fossil Fuel Biodegradation: Fate of [1- 13 C]Acenaphthene in Creosote Polycyclic Aromatic Compound Mixtures Degraded by Bacteria
- Source :
- Applied and Environmental Microbiology. 64:1447-1453
- Publication Year :
- 1998
- Publisher :
- American Society for Microbiology, 1998.
-
Abstract
- [1- 13 C]acenaphthene, a tracer compound with a nuclear magnetic resonance (NMR)-active nucleus at the C-1 position, has been employed in conjunction with a standard broad-band-decoupled 13 C-NMR spectroscopy technique to study the biodegradation of acenaphthene by various bacterial cultures degrading aromatic hydrocarbons of creosote. Site-specific labeling at the benzylic position of acenaphthene allows 13 C-NMR detection of chemical changes due to initial oxidations catalyzed by bacterial enzymes of aromatic hydrocarbon catabolism. Biodegradation of [1- 13 C]acenaphthene in the presence of naphthalene or creosote polycyclic aromatic compounds (PACs) was examined with an undefined mixed bacterial culture (established by enrichment on creosote PACs) and with isolates of individual naphthalene- and phenanthrene-degrading strains from this culture. From 13 C-NMR spectra of extractable materials obtained in time course biodegradation experiments under optimized conditions, a number of signals were assigned to accumulated products such as 1-acenaphthenol, 1-acenaphthenone, acenaphthene-1,2-diol and naphthalene 1,8-dicarboxylic acid, formed by benzylic oxidation of acenaphthene and subsequent reactions. Limited degradation of acenaphthene could be attributed to its oxidation by naphthalene 1,2-dioxygenase or related dioxygenases, indicative of certain limitations of the undefined mixed culture with respect to acenaphthene catabolism. Coinoculation of the mixed culture with cells of acenaphthene-grown strain Pseudomonas sp. strain A2279 mitigated the accumulation of partial transformation products and resulted in more complete degradation of acenaphthene. This study demonstrates the value of the stable isotope labeling approach and its ability to reveal incomplete mineralization even when as little as 2 to 3% of the substrate is incompletely oxidized, yielding products of partial transformation. The approach outlined may prove useful in assessing bioremediation performance.
- Subjects :
- Fossil Fuels
Magnetic Resonance Spectroscopy
Naphthalenes
Applied Microbiology and Biotechnology
law.invention
chemistry.chemical_compound
Bioremediation
Nuclear magnetic resonance
law
Pseudomonas
Methods
Soil Pollutants
Organic chemistry
Polycyclic Aromatic Hydrocarbons
Creosote
Soil Microbiology
Naphthalene
chemistry.chemical_classification
Carbon Isotopes
Bacteria
Ecology
Acenaphthenes
Chemistry
Acenaphthene
Substrate (chemistry)
Mineralization (soil science)
Biodegradation
Biodegradation, Environmental
Hydrocarbon
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 10985336 and 00992240
- Volume :
- 64
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology
- Accession number :
- edsair.doi.dedup.....54befe5c2df1e3037375cdc2d3de0c0a