Back to Search
Start Over
FRET-based fluorescent probe for drug assay from amino acid@gold-carbon nanoparticles
- Source :
- Analytical and bioanalytical chemistry. 413(4)
- Publication Year :
- 2020
-
Abstract
- Biocompatible and luminescent nanostructures synthesized by capping gold-carbon nanoparticles (HOOC-4-C6H4-AuNPs) with amino acids tyrosine, tryptophan, and cysteine were used for the quantitative estimation of ranitidine (RNH), a peptic ulcer and gastroesophageal reflux drug. We applied a fluorescence quenching mechanism to investigate the viability of the energy transfer based on gold-carbon nanosensors. Forster resonance energy transfer (FRET) calculations showed a donor–acceptor distance of 1.69 nm (Tyr@AuNPs), 2.27 nm (Trp@AuNPs), and 2.32 nm (Cys@AuNPs). The constant time-resolved fluorescence lifetime measurements supported the static quenching nature. This method was successfully utilized in the detection and quantification of RNH, with a limit of detection (LOD) of 0.174, 0.56, and 0.332 μM for Tyr@AuNP, Trp@AuNP, and Cys@AuNP bioconjugates, respectively. This approach was also successful in the quantification of RNH in spiked serum samples.
- Subjects :
- Nanoparticle
02 engineering and technology
Ranitidine
01 natural sciences
Biochemistry
Analytical Chemistry
Limit of Detection
Fluorescence Resonance Energy Transfer
Humans
Amino Acids
Fluorescent Dyes
Detection limit
chemistry.chemical_classification
Quenching (fluorescence)
Chemistry
010401 analytical chemistry
Tryptophan
021001 nanoscience & nanotechnology
Anti-Ulcer Agents
Fluorescence
Carbon
0104 chemical sciences
Amino acid
Förster resonance energy transfer
Nanoparticles
Gold
0210 nano-technology
Luminescence
Nuclear chemistry
Subjects
Details
- ISSN :
- 16182650
- Volume :
- 413
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Analytical and bioanalytical chemistry
- Accession number :
- edsair.doi.dedup.....54bc769eaf205419e7feeab651c43de3