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Isolation and expansion of allogeneic myeloma-specific interferon-gamma producing T cells for adoptive immunotherapy
- Publication Year :
- 2006
-
Abstract
- Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4(+) and CD8(+) T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 x 106 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)-based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
- Subjects :
- CD4-Positive T-Lymphocytes
Cancer Research
Time Factors
medicine.medical_treatment
T cell
T-Lymphocytes
Cell Culture Techniques
Succinimides
Cell Separation
Biology
CD8-Positive T-Lymphocytes
Medical Oncology
Immunotherapy, Adoptive
03 medical and health sciences
Interleukin 21
Interferon-gamma
Magnetics
0302 clinical medicine
medicine
Cytotoxic T cell
Humans
Transplantation, Homologous
Interferon gamma
Multiple myeloma
030304 developmental biology
0303 health sciences
Hematology
General Medicine
Immunotherapy
Multiple myeloma, immunotherapy, interferon-gamma, T cell, CFSE
medicine.disease
Fluoresceins
In vitro
3. Good health
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Immunology
Cancer research
Multiple Myeloma
CD8
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....54b6f0f2e87e848c189799c6d47bdfdb