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RelB regulates basal and proinflammatory induction of conjunctival CCL2
- Source :
- Ocular Immunology and Inflammation. 29:29-42
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- Purpose: This study investigated the involvement of NF-kB in regulating postoperative conjunctival inflammation.Methods: Experimental surgery was performed as described for the mouse model of conjunctival scarring. Expression of NF-κB in postoperative conjunctival tissues or conjunctival fibroblasts were assessed by real-time PCR, immunoblotting and immunofluorescence analyses. Downregulation of RelB was achieved using small interfering RNA. Cellular cytokine secretion was determined using multiplex cytokine assay.Results: RelB was the most highly induced member of the NF-kB family on day 2 post-surgery. Elevated RelB may be found associated with vimentin-positive cells and fibroblasts in vivo and in vitro. In conjunctival fibroblasts, RelB may be induced by TNF-α but not TGF-β2 while its silencing caused selective induction of CCL2 secretion by both basal and TNF-α-stimulated fibroblasts.Conclusions: High RelB induction in the inflammatory phase and the selective modulation of CCL2 suggest a specific anti-inflammatory role for RelB in the postoperative conjunctiva.
- Subjects :
- Small interfering RNA
medicine.medical_treatment
Inflammation
Ophthalmologic Surgical Procedures
CCL2
Proinflammatory cytokine
Mice
03 medical and health sciences
Postoperative Complications
0302 clinical medicine
Downregulation and upregulation
Animals
Immunology and Allergy
Medicine
Cells, Cultured
Chemokine CCL2
030203 arthritis & rheumatology
business.industry
RELB
Transcription Factor RelB
Fibroblasts
Conjunctivitis
Mice, Inbred C57BL
Disease Models, Animal
Ophthalmology
Cytokine
Gene Expression Regulation
030221 ophthalmology & optometry
Cancer research
Cytokines
RNA
Cytokine secretion
medicine.symptom
business
Conjunctiva
Subjects
Details
- ISSN :
- 17445078 and 09273948
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Ocular Immunology and Inflammation
- Accession number :
- edsair.doi.dedup.....549cd2bf7436247d9b6a115862b18002