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Cerebrospinal fluid compartmentalization of HIV-1 and correlation with plasma viral load and blood-brain barrier damage
- Source :
- Infection. 47(3)
- Publication Year :
- 2018
-
Abstract
- We aimed to evaluate HIV-1 compartmentalization between the cerebrospinal fluid (CSF) and plasma and investigate as to which extent HIV-1 strains in CSF differ from those in blood and whether a correlation with either plasma viral load (pVL) or an altered blood-brain barrier (BBB) does exist.We retrospectively evaluated paired CSF/blood samples collected from 86 HIV+ patients. HIV-RNA quantification, pol (PR/RT), and V3 sequencing were performed. HIV coreceptor tropism (CRT) was inferred (g2p, false-positive rate 10%, FPR). Data of standard CSF analysis were also reviewed; an altered CSF/plasma albumin ratio signified BBB damage. Neurological abnormalities (NA) were recorded.Overall, 32% of patients had a CSF/plasma HIV-RNA ratio 1 (discordance); 3% of patients had detectable CSF HIV-RNA despite suppressed pVL (escape). Discordance was more frequent in ART-treated patients (p 0.001) and in patients with NA (p = 0.016), but was independent of BBB damage (p = 0.65) and AIDS diagnosis (p = 0.96). Finally, CSF/plasma discordance was significantly more frequent (p 0.0001) in patients with lower pVL values ( 10.000 copies/ml). Env divergence 10% was found in 44% of sequences and was associated with ART (p = 0.008) and NA (p = 0.037). Overall, 24% of patients had a discordant CSF/blood CRT. A 100% nucleotide identity was observed in only 7.3% of pol sequences; notably, 10% of patients had resistance-associated mutations in CSF, but not in blood.Our data confirm an independent replication and evolution of HIV within the CSF. A number of factors either hinder or contribute to the compartmentalization of HIV.
- Subjects :
- Microbiology (medical)
Adult
Male
Human immunodeficiency virus (HIV)
HIV Infections
Blood–brain barrier
medicine.disease_cause
Plasma viral load
Correlation
Plasma
Cerebrospinal fluid
medicine
Humans
Retrospective Studies
business.industry
General Medicine
Compartmentalization (psychology)
Middle Aged
Viral Load
Infectious Diseases
medicine.anatomical_structure
Blood-Brain Barrier
Immunology
HIV-1
Csf analysis
Female
Plasma Albumin
business
Subjects
Details
- ISSN :
- 14390973
- Volume :
- 47
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Infection
- Accession number :
- edsair.doi.dedup.....54947c5ff50f8f8149215e025b0f80b0