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2,3,5,4'-tetrahydroxystilbene-2-O-b-D-glucoside triggers the pluripotent-like possibility of dental pulp stem cells by activating the JAK2/STAT3 axis: Preliminary observations
- Source :
- Journal of Dental Sciences, Journal of Dental Sciences, Vol 16, Iss 2, Pp 599-607 (2021)
- Publication Year :
- 2020
-
Abstract
- Background/Purpose Although 2,3,5,4′-Tetrahydroxystilbene-2-O-beta-glucoside (THSG) reportedly has anti-inflammatory properties, its role in inducing the dedifferentiation of human dental pulp stem cells (DPSC) into pluripotent-like stem cells remains to be determined. The purpose of this study is to evaluate the effects of THSG on the pluripotent-like possibility and mechanism of DPSC. Materials and methods DPSCs were treated with THSG, and cell viability was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTS) assay. Real-time polymerase chain reaction was used to analyze the mRNA expression levels of pluripotency-associated genes and oncogenes and to detect telomerase activity in the cells. Embryoid body formation assay was conducted, and pluripotency-related proteins were identified using Western blotting. Data were analyzed using one-way analysis of variance. Results Cell viability, telomerase activity, and embryoid body formation were enhanced in THSG-treated DPSCs. The mRNA expression levels of pluripotent-like genes (including Nanog homeobox [NANOG], SRY-box 2 [SOX2], and POU class 5 homeobox 1 [POU5F1/OCT4]) significantly increased after THSG treatment. The expression levels of pluripotency-related genes (Janus kinase-signal transducer 2 [JAK2] and signal transducer and activator of transcription 3 [STAT3]) increased, whereas those of oncogenes (Ras, SRC, HER2, and C-sis) decreased. Furthermore, the expression levels of the phosphorylated JAK2 and STAT3 proteins significantly increased after THSG treatment. Conclusion THSG treatment may enhance the pluripotent-like possibility of DPSC through the JAK2/STAT3 axis. Hence, it may be used as an alternative cell-based therapeutic strategy in regenerative dentistry.
- Subjects :
- Homeobox protein NANOG
Pluripotency
Telomerase
2,3,5,4′-tetrahydroxystilbene-2-O-b-glucoside
Chemistry
Cell
Dental pulp stem cells
030206 dentistry
Embryoid body
Cell biology
lcsh:RK1-715
03 medical and health sciences
0302 clinical medicine
medicine.anatomical_structure
SOX2
lcsh:Dentistry
030220 oncology & carcinogenesis
medicine
Original Article
Viability assay
Stem cell
General Dentistry
Subjects
Details
- ISSN :
- 22138862
- Volume :
- 16
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of dental sciences
- Accession number :
- edsair.doi.dedup.....548bd3964b6bb97f57df699acca0be13