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A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019), Gioacchini, G, Marisaldi, L, Basili, D, Candelma, M, Pignalosa, P, Aiese Cigliano, R, Sanseverino, W, Hardiman, G & Carnevali, O 2019, ' A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius) ', Scientific Reports, vol. 9, no. 1, 7375 . https://doi.org/10.1038/s41598-019-43872-6
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- The Mediterranean swordfish (Xiphias gladius) has been recently classified as overfished and in 2016, the International Commission for the Conservation of the Atlantic Tunas (ICCAT) established a multi-annual management plan to recover this stock. To successfully achieve this goal, knowledge about swordfish biology is needed. To date, few studies on swordfish have been performed and none of them has provided useful insights into the reproductive biology at molecular level. Here we set to characterise the molecular dynamics underlying ovarian maturation by employing a de novo transcriptome assembly approach. Differential gene expression analysis in mature and immature ovaries identified a number of differentially expressed genes associated with biological processes driving ovarian maturation. Focusing on ovarian steroidogenesis and vitellogenin uptake, we depict the molecular dynamics characterizing these processes while a phylogenetic analysis let us identify a candidate vitellogenin receptor. This is the first swordfish transcriptome assembly and these findings provide in-depth understanding of molecular processes describing ovarian maturation. Moreover, the establishment of a publicly available database containing information on the swordfish transcriptome aims to boost research on this species with the long-term of developing more comprehensive and successful stock management plans.
- Subjects :
- 0301 basic medicine
Conservation of Natural Resources
De novo transcriptome assembly
lcsh:Medicine
Receptors, Cell Surface
Biology
Article
Transcriptome
Vitellogenins
03 medical and health sciences
Vitellogenin
0302 clinical medicine
Molecular level
Animals
Sexual Maturation
Gladius
General
lcsh:Science
Transcriptomics
Phylogeny
Multidisciplinary
Gene Expression Profiling
Swordfish
lcsh:R
Egg Proteins
Ovary
Fishes
Gene Expression Regulation, Developmental
Molecular Sequence Annotation
030104 developmental biology
Differentially expressed genes
Evolutionary biology
biology.protein
lcsh:Q
Female
Steroids
Ichthyology
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....5478818bdb846a19b52f5ace53f50262